MicroPET/CT-based exploration of the effects of acute sleep deprivation on glucose metabolism and neuroinflammation in rat brain
10.3760/cma.j.cn321828-20241101-00375
- VernacularTitle:基于microPET/CT探讨急性睡眠剥夺对大鼠脑内葡萄糖代谢及神经炎性反应的影响
- Author:
Mengya DAI
1
;
Zhenyu XIANG
;
Yan ZHANG
;
Chaofeng LIU
;
Jie GAO
;
Zhixing QIN
;
Hongliang WANG
;
Zhifang WU
;
Jianguo LI
;
Sijin LI
Author Information
1. 山西医科大学第一医院核医学科、分子影像山西省重点实验室、分子影像精准诊疗省部共建协同创新中心，太原 030001
- Publication Type:Journal Article
- Keywords:
Sleep deprivation;
Hippocampus;
Metabolism;
Glucose;
Neuroinflammatory diseases;
Positron-emission tomography;
Tomography, X-ray computed;
Rats
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2025;45(9):555-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of acute sleep deprivation (ASD) on hippocampal glucose metabolism and neuroinflammation in rat models.Methods:Twenty SD rats (10 males and 10 females) were divided into four groups (five in each group) by random sampling method: female ASD group, male ASD group, female control group, and male control group. Among them, the ASD group constructed the ASD model. After 72h sleep deprivation, all rats underwent 18F-FDG and N, N-diethyl-2-(2-(4-(2- 18F-fluoroethoxy)phenyl)-5, 7-dimethylpyrazolo[1, 5-a]pyrimidin-3-yl)acetamide ( 18F-DPA-714) microPET/CT brain imaging in 2d to compare the changes of 18F-FDG and 18F-DPA-714 SUV mean in the hippocampus of rats. Brain histopathology, immunohistochemistry and immunofluorescence staining were detected in rats. Independent-sample t test was used to analyze the data. Results:18F-FDG imaging showed the hippocampal SUV mean between ASD group and control group (female: 4.11±0.35 vs 1.89±0.28; male: 3.43±0.47 vs 2.02±0.54) were statistically significant ( t values: 9.65, 3.92, P values: <0.001, 0.002). 18F-DPA-714 imaging showed the hippocampal SUV mean between ASD group and control group (females: 0.28±0.01 vs 0.28±0.02; male: 0.26±0.02 vs 0.31±0.04) were not statistically significant ( t values: -0.18, -2.24, P values: 0.859, 0.056). The 18×10 3 translocator protein (TSPO) immunohistochemistry showed the expression in the hippocampal region of the brain between ASD group and control group (female: 0.19±0.02 vs 0.19±0.01; male: 0.21±0.01 vs 0.20±0.01) were not statistically different ( t values: -0.48, -1.67, P values: 0.651, 0.139). Immunofluorescence staining showed that microglial cytosol in the hippocampal region of the brain decreased after 72h of ASD, and the protrusion points and surrounding branches were significantly reduced. Conclusion:Increased hippocampal glucose metabolism in rats is observed after 72 h of ASD without significant neuroinflammation.
