Roles of IgG1 and IgG2a subtypes in anti-glycoprotein Ⅰbα antibody-induced platelet clearance
10.3760/cma.j.cn121090-20240710-00256
- VernacularTitle:血小板糖蛋白Ⅰbα抗体IgG1和IgG2a亚型在诱导血小板清除中的作用
- Author:
Sai ZHANG
1
;
Yue XIA
1
;
Honglei YE
1
;
Kangxi ZHOU
1
;
Chenglin SUN
1
;
Mengxing CHEN
1
;
Kesheng DAI
1
;
Rong YAN
1
Author Information
1. 苏州大学医学院，苏州大学附属第一医院，江苏省血液研究所，国家卫健委血栓与止血重点实验室，苏州 215006
- Publication Type:Journal Article
- Keywords:
Glycoprotein Ⅰbα;
IgG2a;
Thrombocytopenia
- From:
Chinese Journal of Hematology
2025;46(6):558-563
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of the IgG subtypes (IgG1 and IgG2a) in anti-glycoprotein (GP) Ⅰbα antibody-induced platelet clearance.Methods:Venous blood was collected from healthy volunteers, and platelets were separated. The phagocytosis of human platelets by human acute monocytic leukemia cells (THP-1 cells) induced by different anti-GPⅠbα antibodies (AN51, AK2, HIP1, TM60, VM16d, WM23, and SZ2) was detected by flow cytometry. The effects of the AN51 full-length antibody, F (ab') 2, and Fab fragments on platelet phagocytosis by THP-1 cells were detected by flow cytometry. Then, the Fc blocking antibody 2.4G2 and normal rat IgG2a or IgG1 were injected into C57BL/6J mice via the posterior ocular vein, and their effects on platelet reduction induced by R300 were detected by a hematology analyzer. Results:Compared with IgG1, the IgG2a subtype of anti-GPⅠbα antibodies induced the phagocytosis of platelets by THP-1 cells in vitro ( P<0.05). In contrast to the AN51 full-length antibody, neither AN51 F (ab') 2 nor the Fab fragment could induce THP-1 cells to phagocytose platelets ( P<0.05). Compared with the control group, anti-mouse GPⅠbα R300-induced thrombocytopenia in mice was reduced at 2, 4, and 6 h after the injection of Fc blocking antibody 2.4G2 ( P<0.05). Similarly, R300-induced thrombocytopenia in mice was reduced at 2, 4, and 6 h after the injection of rat IgG2a ( P<0.05) . Conclusion:IgG2a plays an important role in anti-GPⅠbα-induced clearance.
