In vitro study of ultrasound/near-infrared bimodal responsive quantum dots nanomolecular probes for microthrombosis diagnosis and treatment
10.3760/cma.j.cn321828-20240227-00070
- VernacularTitle:超声/近红外双模态响应型量子点纳米探针用于微血栓诊疗的体外实验研究
- Author:
Zhixin JIANG
1
;
Nan JIANG
;
Zhiwen WANG
;
Mengying SUN
;
Bin GUI
;
Zhiquan TIAN
;
Bo HU
Author Information
1. 武汉大学人民医院超声影像科，武汉 430060
- Publication Type:Journal Article
- Keywords:
Thrombosis;
Nanoparticles;
Quantum dots;
Ultrasonography;
Spectroscopy, near-infrared;
Mice, nude;
Rats
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2025;45(1):29-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the application potential of the bimodal ultrasound/near-infrared (NIR) composite nanoscale probe Arg-Gly-Asp (RGD)/Ag 2Te/perfluoropentane (PFP) @ mesoporous silica nanoparticles (MSN) in the diagnosis and treatment of microvascular diseases. Methods:Nanoprobes loaded with RGD, PFP and Ag 2Te were prepared by ultrasound sonication and carbodiimide method. The characterization of the nanoprobes was determined. The imaging performance, photothermal response, and target-seeking ability of the nanoprobes under NIR irradiation were verified. The biosafety of the nanoprobes was examined, and the thrombolytic ability of the nanoprobes was evaluated. The mice were observed to visualize microvessels of the abdominal wall under the NIR-Ⅱ imaging, and the microvascular visualization ability of the nanoprobes was evaluated. Results:The particle size of nanoprobes was (205.3±2.9) nm and the potential was (2.05±0.58) mV. The coupling rate of the RGD was (82.27±0.36)%, the encapsulation rate of the quantum dots was (80.80±3.26)%, and the photostability of the quantum dots was good. The fluorescence intensity was enhanced with the increase of the mass concentration of RGD/Ag 2Te/PFP@MSN, and the warming effect was more obvious. After ultrasound and NIR irradiation, the thrombolysis rate was significantly increased. RGD/Ag 2Te/PFP@MSN successfully realized NIR-Ⅱ fluorescence imaging of mice microvessels. The cytotoxicity assay and hemolysis assay showed that the probe had a good biosafety. Conclusion:The RGD/Ag 2Te/PFP@MSN nanoprobe is a potential strategy for targeted therapy of thrombotic diseases, combining dual-modality therapy of ultrasound and NIR to offer new possibilities for non-invasive and visual diagnosis and treatment of microvascular embolism.
