Molecular epidemiology study of Enterobacteriales carrying blaNDM gene in Lishui area
10.3760/cma.j.cn112309-20241107-00379
- VernacularTitle:丽水地区携带 blaNDM肠杆菌目细菌的分子流行病学研究
- Author:
Jiaoli CHEN
1
;
Zhiming GONG
1
;
Jianfen XU
1
;
Xiaopeng LIU
1
;
Shiqi FAN
1
;
Yun′an ZHAO
1
;
Xinmi ZHAO
1
;
Xiaolei HU
1
;
Jiansheng HUANG
1
Author Information
1. 温州医科大学附属第五医院/丽水市中心医院检验科，丽水 323000
- Publication Type:Journal Article
- Keywords:
Enterobacter;
blaNDM;
Drug resistance characteristics;
Molecular epidemiological characteristics
- From:
Chinese Journal of Microbiology and Immunology
2025;45(4):317-323
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the drug-resistance pattern and molecular epidemiological characteristics of Enterobacteriales carrying the blaNDM gene in Lishui, aiming to guide clinical anti-infection treatment. Methods:Non-duplicate blaNDM-carrying Enterobacteriales, isolated from Lishui Central Hospital, were collected and identified by VITEK MS. The minimal inhibitory concentrations (MICs) were detected by the broth microdilution method. The ST types of the strains were determined by multilocus sequence typing (MLST). Plasmid types were identified by transformation or conjugation experiments and replication initiator amplification experiments. The transposon structures were detected by PCR amplification. Finally, the epidemic regularity of blaNDM gene in Lishui was analyzed from three levels: clonal group, plasmid, and mobile genetic elements. Results:A total of 109 blaNDM-positive strains were collected. Among them, 60 strains carried the blaNDM-1 gene and 49 strains carried the blaNDM-5 gene. The 109 strains showed 100% resistance to ceftazidime and cefotaxime. The resistance rates to peracillin-tazobactam and imipenem were higher than 80%. Strains carrying the blaNDM-5 gene were more resistant to meropenem than those carrying blaNDM-1 gene( P<0.05). A total of 68 STs were detected from 109 strains, and IncX3, IncFⅡγ, IncA/C and IncT/R plasmids were detected, and 90.83% of the blaNDM genes were located in the IncX3 plasmid. Twelve types of blaNDM gene surrounding structures existed, and they all carried the highly conserved blaNDM- bleMBL- trpF gene sequence. Conclusions:The blaNDM gene has diverse transmission modes in Lishui. The IncX3 plasmid is the main factor mediating its transfer, and all strains carry highly conserved blaNDM- bleMBL- trpF gene sequence.
