Construction and evaluation of a standard strain candidate of F genotype mumps virus
10.3760/cma.j.cn112866-20250325-00067
- VernacularTitle:F基因型流行性腮腺炎病毒标准株候选株的构建和评价
- Author:
Yan TANG
1
;
Xiongwei XU
;
Xunmin JI
;
Xiaofang PENG
;
Changwen KE
;
Lei ZHANG
Author Information
1. 广东省生物制品与药物研究所，广州 510440
- Publication Type:Journal Article
- Keywords:
Mumps virus;
Plaque purification;
Standard strain
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(5):604-610
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a monoclonal strain of mumps virus of genotype F，which is intended to be applied as a national standard strain，and to provide corresponding virus strain resources for the surveillance，prevention and control of mumps.Methods:The plaque purification technique was used to pick monoclonal strains on Vero/hSLAM cells. The biological characteristics and stability of the monoclonal virus strains were evaluated by observing the cytopathic effect of the monoclonal virus strains，morphological observation of virological characteristics，determination of virus titer and whole-genome sequencing，and mycoplasma detection.Results:After infection of Vero/hSLAM cells，the monoclonal strain of mumps virus constructed after plaque purification could cause cell fusion. Under the negative staining electron microscope，the virus particles were spherical，with a diameter between 100 and 200 nm，and had an obvious envelope. The virus titer of the 2 nd to 6 th generations was between 10 6 and 10 7 TCID 50/ml. Through whole-genome sequencing and phylogenetic analysis，the full length of the virus genome sequence was obtained as 15 384 bp，and it was confirmed that the monoclonal virus strain was of genotype F. The nucleotide difference rate between the 2 nd and 6 th generations was 0.013%. Conclusion:The monoclonal strain of mumps virus constructed in this experiment has typical cytopathic effects and typical morphological structures. The virus has good activity and stable genetic characteristics，and can be used as a candidate strain for application as a national standard strain. It can be used for reagent research and development，evaluation of immunization effects，and other subsequent work.
