Euphorbia Lathyrol effects expressions of immune factors IL-7,IL-10 and VEGF in renal clear cell neoplasias mice through TGF-β/Smad signaling pathway
10.3969/j.issn.1000-484X.2025.07.007
- VernacularTitle:千金子二萜醇通过TGF-β/Smad信号通路影响肾透明细胞癌小鼠免疫因子IL-7、IL-10及VEGF表达
- Author:
Shengyou SONG
1
;
Lei ZHOU
;
Lunwei TAI
;
Shijie PAN
;
Junling JIANG
;
Junfeng ZHAO
Author Information
1. 河南中医药大学第二临床医学院,郑州 450002
- Publication Type:Journal Article
- Keywords:
Euphorbia Lathyrols;
Renal clear cell neoplasias;
TGF-β/Smad signal transduction systems;
IL-7;
IL-10;
VEGF
- From:
Chinese Journal of Immunology
2025;41(7):1581-1585,1592
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Euphorbia Lathyrol on expressions of IL-7,IL-10 and VEGF in renal clear cell neoplasias(RCCN)mice by regulating TGF-β/Smad signaling pathway.Methods:RCCN xenograft mice were established and divided into model group,Euphorbia Lathyrol group and Cisplatin group.Euphorbia Lathyrol group were given 20 mg/kg Euphorbia Lathyrol solution intragastric administration,model group were given 0.9%NaCl gastric feeding,Cisplatin group were given intraperito-neal injection of 4 mg/kg cisplatin solution,then mice weight and tumor volume were recorded.Expressions of TGF-β1,Smad2 and Smad4 were tracked down through Western blot.Expressions of immune factors IL-7 and IL-10 in serum of xenograft mice were detected by ELISA.Expression of VEGF in tumor mass was detected by IHC.Results:After 14 days of administration,compared with model group,the proliferation of tumor grafts in Cisplatin group and Euphorbia Lathyrol group was inhibited(P<0.05),and the expressions of TGF-β1,Smad2,Smad4 protein and VEGF were observably decreased(P<0.05),expressions of IL-7 in serum was increased,while level of IL-10 in serum was decreased(P<0.05).Conclusion:Euphorbia Lathyrol is able to reduce expressions of IL-7,IL-10,as well as VEGF in RCCN xenograft mice by impeding TGF-β1/Smad signaling transduction system.
