Huanglian Jiedu Decoction:A traditional Chinese medicine recipe for disrupting platelet-neutrophil cross-talk and regulating inflammatory response
10.3969/j.issn.1000-484X.2025.07.029
- VernacularTitle:黄连解毒汤:减少炎症反应中血小板-中性粒细胞串扰的中药配方
- Author:
Miao WANG
1
;
Fenglin ZHU
;
Jingru CHEN
Author Information
1. 重庆市中医院,重庆 400021
- Publication Type:Journal Article
- Keywords:
Huanglian Jiedu Decoction;
Platelet-neutrophil cross-talk;
Inflammatory response
- From:
Chinese Journal of Immunology
2025;41(7):1743-1751
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of Huanglian Jiedu Decoction(HLJDD)on platelet-neutrophil crosstalk in inflammatory response.Methods:The rat model of inflammatory reaction was induced by lipopolysaccharide(LPS),and HLJDD was used to intervene LPS rats.Proinflammatory factors and platelet-neutrophil cross-talk were detected.Immunofluorescence and HE staining were used to observe the platelet-neutrophil adhesion rate in lung tissue and lung histopathology.Transcriptomics was used to explore the potential signaling pathways and targets of HLJDD in platelet-neutrophil crosstalk and verified them.Results:Com-pared with the control group,the white blood cell count,platelet count,plasma TNF-α,IL-1β,SLEP,sC5b-9 and ICAM-1 levels in the model group were significantly increased,and the positive expression rates of CD62p,MPO and CD61+CD62p+MPO coincidence in the lung tissue of the model group were also significantly increased(P<0.001 or P<0.05).The inflammatory injury was severe in the model group.Compared with the model group,the white blood cell count,plasma levels of TNF-α,IL-1β,SLEP,sC5b-9 and ICAM-1 in the HLJDD group were decreased(P<0.001 or P<0.05),the positive expression rates of CD62p and MPO and the coincident posi-tive expression rates of CD61+CD62p+MPO in the lung tissue of the HLJDD group were decreased(P<0.05),and LPS-induced lung tis-sue injury was alleviated.Transcriptomics results showed that high-dose HLJDD could down-regulate differential genes on oxidative phosphorylation pathways.Conclusion:HLJDD ameliorates LPS-induced inflammatory response and reduces platelet-neutrophil cross-talk,which may be related to the down-regulation of oxidative phosphorylation.
