Research on etiological sampling for large-scale object surfaces in hospitals based on enrichment technology
10.11816/cn.ni.2025-250895
- VernacularTitle:基于富集技术的医院大面积物表病原采样处理方法
- Author:
Jianming ZHOU
1
;
Yujuan JIN
;
Miaoer LAI
;
Haiduan LIN
;
Ao LUO
;
Jingmei LI
;
Yingjian CHEN
;
Qiongyao WANG
;
Zeren LIN
;
Xiaoli WANG
Author Information
1. 深圳市龙岗区疾病预防控制中心微生物检验科,广东 深圳 518172
- Publication Type:Journal Article
- Keywords:
Etiological surveillance of hospital environment;
Anti-static fabric;
Polyethylene glycol precipitati-on method;
Swab;
Enrichment concentration;
Sampling
- From:
Chinese Journal of Nosocomiology
2025;35(22):3495-3499
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To compare the effect of traditional swab sampling method on etiological surveillance of in-fectious diseases for large-scale object surfaces in hospitals and validate the samples processing method based on pre-wet anti-static fabric and modified polyethylene glycol(PEG)precipitation enrichment technology so as to im-prove the capability of early warning of infectious diseases and optimize the environmental surveillance program in the hospitals.METHODS The on-site surveillance was carried out for 8 times in 3 public hospitals(Shenzhen Longgang People's Hospital,the Second People's Hospital of Longgang and Longgang Maternal and Child Health Hospital)from May 2024 to Mar.2025.Totally 23 types of respiratory tract pathogens(18 types of viruses,5 type of pathogenic bacteria)and 6 types of gastrointestinal tract pathogens were simultaneously detected by means of fluorescent quantitative polymerase chain reaction(PCR);the actual isolation rates,etiological spectrum and cycle threshold(Ct)value were compared.The acrylic plate added with standards of different loads of H1NI influ-enza viruses was used as model for laboratory evaluation.The minimum detection limit,sensitivity and repeatabili-ty were observed and compared between the methods.RESULTS The minimum detection limit of both methods was 6.0 × 104 copies/ml,however,the positive rate of nucleic acid testing of the pre-wet fabric method was 100.00％(3/3),higher than 33.33％(1/3)of the swab method;when the low viral load was 6.0× 105 copies/ml,the average concentration of viral nucleic acid of the pre-wet fabric method(X-Ct=36.59)was higher,with the re-peatability(CV=0.99％,＜3.14％)better.The results of the on-site surveillances showed that the total isolation rates of pathogens of the pre-wet fabric method ranged between 42.84％and 64.27％,higher than between 10.71％and 21.43％of the swab method,with the isolated pathogens more abundant,the Ct value lower(P＜0.05).CONCLUSION The pre-wet fabric sampling enrichment method integrated with anti-static fabric sampling and PEG enrichment technology shows higher sensitivity and stability in the etiological surveillance of large-scale object surfaces,raising the isolation rate.
