Rutin promoting the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth
10.12007/j.issn.0258-4646.2025.04.015
- VernacularTitle:芦丁促进人乳牙牙髓干细胞的增殖及成骨分化
- Author:
Ni FENG
1
;
Yuehong LI
1
Author Information
1. 长沙市第四医院口腔科,长沙 410006
- Publication Type:Journal Article
- Keywords:
rutin;
Janus protein tyrosine kinase 2;
signal transducer and activator of transcription 3;
stem cells from human exfoliated deciduous teeth;
proliferation;
osteogenic differentiation
- From:
Journal of China Medical University
2025;54(4):369-374
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of rutin on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHEDs)and its regulatory relationship with the Janus protein tyrosine kinase 2(JAK2)/signal transducer and activator transcription 3(STAT3)pathway.Methods SHEDs were isolated and cultured in vitro.After identification,SHEDs were randomly grouped into the control group(normal culture),low,medium,and high dose rutin groups(0.1,0.5,1 μmol/L),and the inhibi-tor group(1 μmol/L of rutin+10 μmol/L of the JAK2/STAT3 pathway inhibitor AG490).Alizarin red staining was applied to detect the number of mineralized nodules,and the MTT method was utilized to detect cell proliferation.A commercial kit was used to detect cell alkaline phosphatase(ALP)activity,and quantitative real-time PCR was applied to detect the mRNA expression of Runt-related transcrip-tion factor 2(Runx2),osteocalcin(OCN),and alkaline phosphatase(ALP).Western blotting was used to detect the expression of Runx2,OCN,ALP,and JAK2/STAT3 signaling pathway proteins.Results Compared to that of the control group,the optical density value of SHEDs cells,the number of mineralized nodules,ALP activity,Runx2,OCN,ALP mRNA and protein levels,and p-JAK2/JAK2 p-STAT3/STAT3 were all markedly increased in the low,medium,and high dose rutin groups(P＜0.05).Treatment with the JAK2/STAT3 pathway inhibitor AG490 attenuated the enhancement of rutin on the proliferation and osteogenic differentiation of SHEDs(P＜0.05).Conclu-sion Rutin promotes the proliferation and osteogenic differentiation of SHEDs,and its mechanism is related to the activation of the JAK2/STAT3 signaling pathway.
