Protective effect of 4-hydroxybenzyl alcohol on ischemic astrocytes based on energy metabolism
10.3969/j.issn.1009-0126.2025.11.025
- VernacularTitle:基于能量代谢研究4-羟基苯甲醇对缺血性星形胶质细胞的保护作用
- Author:
Tian XIAO
1
;
Jie TAO
1
;
Jiaoyang TAN
1
;
Zhourong ZHAO
1
;
Xiaohua DUAN
1
Author Information
1. 云南中医药大学云南省傣医药与彝医药重点实验室,昆明 650500
- Publication Type:Journal Article
- Keywords:
gastrodiae rhizoma;
astrocytes;
energy metabolism;
oxygen-glucose deprivation/reoxygenation
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2025;27(11):1558-1561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ameliorative effects of 4-hydroxybenzyl alcohol(4HBA)on energy metabolism in ischemic astrocytes(ASC).Methods Primary ASC were isolated from rat cortical tissue,and then randomly divided into control,oxygen-glucose deprivation/reoxygenation(OGD/R)group,low-,medium-and high-dose 4HBA groups(50,100 and 200 μmol/L),and edaravone group.Except for the control group,OGD/R injury models were established in a tri-gas incubator.The following parameters were measured:cell mortality,adenosine triphosphate(ATP)content,activities of mitochondrial ATP enzymes(Na+/K+-ATPase and Ca2+/Mg2+-ATPase),activities of mitochondrial complexes Ⅰ-Ⅴ,and expression of energy metabolism-related genes.Results Compared with the control group,the OGD/R group showed significantly higher cell mortality[(9.49±0.85)％vs(0.00±0.00)％,P＜0.01],along with obviously decreased ATP content,Na+/K+-ATPase activity,Ca2+/Mg2+-ATPase activity,and mitochondrial complex Ⅰ-V activities(P＜0.01).Compared with the OGD/R group,three 4HBA groups as well as the edaravone group exhibited significantly reduced cell mortality and elevated ATP content,Na+/K+-ATPase activity,and complex Ⅰ,Ⅲ,and V activities.The high-and medium-dose 4HBA groups and the edaravone group demonstrated increased Ca2+/Mg2+-ATPase activity,and the high-dose 4HBA group and edaravone group exhibited more enhanced complexⅡ activity,and the three 4HBA groups had elevated complex Ⅳ activity(P＜0.05,P＜0.01).Conclusion 4HBA exerts protective effect against ischemic injury in ASC by mitigating energy metabolism dysfunction and reducing cell death.
