Preliminary study on the role of peptidyl arginine deiminase 4 in the regulation of maxillofacial development
10.13591/j.cnki.kqyx.2025.04.001
- VernacularTitle:PAD4在颌骨生长发育调控中的作用初探
- Author:
Xingzhi YAN
1
;
Xinyu CAI
1
;
Simai CHEN
1
;
Weiwen FANG
1
;
Fan LEI
1
;
Dan CAO
1
;
Yang ZHANG
1
Author Information
1. 南京医科大学附属口腔医院正畸科,口腔疾病研究与防治国家级重点实验室培育建设点,江苏省口腔转化医学工程研究中心,江苏南京(210029)
- Publication Type:Journal Article
- Keywords:
peptidyl arginine deiminase 4;
mesenchymal stem cells isolated from oral bones;
craniomaxillofacial development
- From:
STOMATOLOGY
2025;45(4):241-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of peptidyl arginine deiminase 4(PAD4)on the differentiation of mesenchymal stem cells isolated from oral bones(OMSC)and craniomaxillofacial development.Methods Immunofluorescence was used to detect the ex-pression of PAD4 in the mandibular of mice E13.5 embryo.A peptidyl arginine deiminase 4 knockout(PAD4-KO)mouse model was constructed.Craniomaxillofacial development was investigated by micro-CT.CCK-8 assay and Transwell assay were used to detect the OMSC proliferation ability and migration ability of PAD4-KO and wild type(WT)mouse.ALP staining was used to detect the changes in OMSC osteogenic differentiation ability.The expression of osteogenesis-related genes was detected by immunofluorescence and PCR assay.Results PAD4 was highly expressed in the mandibular tissue of mouse embryos at E13.5.On the cellular level,PAD4 was ex-pressed in the nucleus and mitochondria of OMSC.Compared to the WT mice,micro-CT showed that PAD4-KO mice had retrusive jaw and decreased mineralization.The proliferation and migration ability of OMSC in PAD4-KO mice were decreased.OMSCs lacking PAD4 had significantly decreased ALP staining level,and the expression levels of osteogenesis-related genes were decreased.In addition,it was found that PAD4 might affect OMSC mineralization by regulating Runx2 transcription.Conclusion PAD4 is expressed in the jaw during embryonic development.It might affect the embryonic development by regulating the proliferation and differentiation of OMSC,leading to craniomaxillofacial abnormalities
