Study of Pollen Typhae extract inhibition of renal tubular epithelial cell injury induced by high glucose through microRNA-148b-3p/nuclear factor-κB pathway
10.3969/j.issn.1006-6187.2025.04.009
- VernacularTitle:蒲黄提取物通过微小核糖核酸-148b-3p/核因子κB通路抑制高糖诱导的肾小管上皮细胞损伤的研究
- Author:
Ye CHEN
1
;
Haiyan YU
1
;
Chonggao JIA
1
;
Ruixue CHEN
1
;
Rui YAN
1
Author Information
1. 550004 贵阳,贵州医科大学附属医院肾内科
- Publication Type:Journal Article
- Keywords:
Pollen Typhae extract;
Renal tubular epithelial cells;
Apoptosis;
miR-148b-3p;
Nuclear factor-κB pathway
- From:
Chinese Journal of Diabetes
2025;33(4):286-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of Pollen Typhae extract on renal tubular epithelial cell(HK-2)injury induced by high glucose through microRNA-148b-3p(miR-148b-3p)/nuclear factor-κB(NF-κB)pathway.Methods HK-2 cells were divided into normal control(NC)group,hypertonic control(MG)group,high glucose(HG)group,low dose Pollen Typhae extract(HG+L)group,medium dose Pollen Typhae extract(HG+M)group,high dose Pollen Typhae extract(HG+H)group,HG+inhibitor negative control(anti-miR-NC)group,HG+miR-148b-3p inhibitor(HG+anti-miR-148b-3p)group,HG+mimic negative control+high-dose Pollen Typhae extract(HG+miR-NC+H)group,HG+miR-148b-3p mimic+high-dose Pollen Typhae extract(HG+miR-148b-3p+H)group.Apoptosis rate was detected by flow cytometry.The levels of tumor necrosis factor α(TNF-α)and interleukin 6(IL-6)were detected by ELISA.Malondialdehyde(MDA)level and superoxide dismutase(SOD)activity were detected by spectrophotometry.Reactive oxygen species(ROS)level was detected by DCFH-DA method.qRT-PCR was used to detect the expression of miR-148b-3p.The expression of phosphorylated nuclear factor κB P65(p-p65)and phosphorylated NF-κB inhibitor(p-IκBα)proteins were detected by western blot.Results Compared with NC group,the apoptosis rate,TNF-α and IL-6 secretion levels,MDA level,ROS level,miR-148b-3p expression,Cleaved caspase-3,p-p65,and p-IκBα protein expression were increased(P＜0.05),while cell survival rate and SOD level were decreased in HG group(P＜0.05).Compared with the HG group,HG+L,HG+M,HG+H group increased cell survival rate,SOD(P＜0.05),apoptosis rate,TNF-α,IL-6,MDA,ROS,miR-148b-3p expression,Cleaved-caspase-3,p-p65,p-IκBα protein expression decreased(P＜0.05).Compared with HG+anti-miR-NC group,the cell survival rate and SOD were increased(P＜0.05),while apoptosis rate,expression of TNF-α,IL-6,MDA,ROS,miR-148b-3p,Cleaved caspase-3,p-p65,and p-IκBα protein expression were decreased in HG+anti-miR-148b-3p group(P＜0.05).Compared with HG+miR-NC+H group,apoptosis rate,TNF-α,IL-6,MDA,ROS,miR-148b-3p expression,Cleaved caspase-3,p-p65,and p-IκBα protein expression were increased(P＜0.05),while cell survival rate and SOD were decreased in HG+miR-148b-3p+H group(P＜0.05).Conclusions Pollen Typhae extract can reduce renal tubular epithelial cell injury induced by high glucose through down-regulating the expression of miR-148b-3p and inhibiting NF-κB pathway.
