Protective effect of Liraglutide inrats with diabetic kidney disease by regulating nuclear factor E2-related factor 2/glutathione peroxidase 4 ferroptosis signaling pathway
10.3969/j.issn.1006-6187.2025.04.011
- VernacularTitle:利拉鲁肽调控核因子E2相关因子2/谷胱甘肽过氧化物酶4铁死亡信号对糖尿病肾脏疾病大鼠保护作用的研究
- Author:
Dong LIANG
1
;
Qianyu LIN
;
Min YANG
;
Mengjie LI
;
Wenhua XING
;
Ning YU
;
Yunqi LIU
;
Xiaomin ZHANG
Author Information
1. 256600 滨州医学院附属医院肾内科
- Publication Type:Journal Article
- Keywords:
Liraglutide;
Diabetic kidney disease;
Ferroptosis;
Nuclear factor erythroid 2-realated factor 2;
Glutathione peroxidase 4
- From:
Chinese Journal of Diabetes
2025;33(4):299-303
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Liraglutide in rats with diabetic kidney disease(DKD)by regulating the nuclear factor E2-related factor 2(Nrf2)/glutathione peroxidase 4(GPX4)ferroptosis signaling pathway.Methods Twelve male Sprague-Dawley(SD)rats were randomly divided into normal control(NC)group,DKD group,and Liraglutide treatment(Lir)group,with 4 rats in each group.The 24 hUAlb,TC,TG,LDL-C,serum creatinine(Scr),BUN,ferrous ion(Fe2+),the activity of glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)were detected in each group.Hematoxylin and eosin(HE),periodic acid-Schiff(PAS),and periodic acid-silver methenamine-Masson(PASM-Masson)staining were used to observe the pathological changes of the kidneys.Immunofluorescence was performed to detect the localization and expression of reactive oxygen species(ROS)in the renal tissue.The protein expressions of Nrf2 and GPX4 were detected by Western blot.Results Compared with the NC group,the levels of 24 hUAlb,Scr,BUN,TC,TG,LDL-C,MDA,ROS,and Fe2+were increased(P＜0.05 or P＜0.01),while the expressions of GSH-Px,Nrf2,and GPX4 proteins were decreased in the DKD group(P＜0.01).Compared with the DKD group,the levels of 24 hUAlb,BUN,TC,TG,LDL-C,MDA,ROS,and Fe2+were decreased(P＜0.05 or P＜0.01),and the expressions of GSH-Px,Nrf2,and GPX4 proteins were increased in the Lir group(P＜0.01).Conclusions Liraglutide may exert a protective effect in DKD by upregulating the Nrf2/GPX4 signaling pathway and inhibiting ferroptosis.
