Effect of adipose-derived stem cell exosome on fibroblasts and wound healing
10.12007/j.issn.0258-4646.2025.03.009
- VernacularTitle:脂肪干细胞源性外泌体对成纤维细胞及烧伤创面愈合的影响
- Author:
Na ZUO
1
;
Kai TAO
1
;
Qi TANG
1
;
Meng YU
1
Author Information
1. 中国医科大学附属第四医院烧伤整形显微外科,沈阳 110012
- Publication Type:Journal Article
- Keywords:
adipose-derived stem cell exosome;
fibroblast;
wound healing
- From:
Journal of China Medical University
2025;54(3):233-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of adipose-derived stem cell exosome(ADSC-Exo)on the proliferation and migration of fibroblasts as well as on wound healing of deep second degree burns in rats.Methods Fibroblasts were cultured with ADSC-Exo or in phosphate-buffered saline(PBS).The effect of ADSC-Exo on the proliferation and migration of fibroblasts was observed using the CCK-8 assay and scratch test.Forty Sprague-Dawley(SD)rats were selected to construct a deep second-degree burn model and divided into the exosome and control groups.The exosome group was injected with the ADSC-Exo solution around the wound,and the control group was injected with an equal amount of PBS solution.Wound healing was observed in both groups.Inflammatory infiltration was observed using hematoxylin and eosin(HE)staining,and wound angiogenesis was observed using CD31 immunohistochemical staining.Results The CCK-8 assay revealed that ADSC-Exo promoted fibroblast proliferation in a concentration-dependent manner.The results of the scratch test showed that ADSC-Exo significantly enhanced the migratory ability of fibroblasts(P＜0.000 1).The wound healing results showed that ADSC-Exo injection promoted burn wound healing in rats(P＜0.000 1).Pathological analysis indicated that inflammatory cell infil-tration was less and CD31 expression was higher in the ADSC-Exo group than in the control group(P＜0.01).Conclusion ADSC-Exo promoted the proliferation and migration of fibroblasts,reduced wound inflammation,promoted neovascularization,and enhanced wound healing.
