Effects of combined imatinib mesylate and sunitinib malate therapy on migration and invasion of gastrointestinal stromal tumor cells
10.12007/j.issn.0258-4646.2025.03.001
- VernacularTitle:甲磺酸伊马替尼和苹果酸舒尼替尼联合应用对胃肠道间质瘤细胞迁移和侵袭能力的影响
- Author:
Zhijian LI
1
;
Wensi WANG
;
Hongying MA
;
Junkai JIA
;
Tianbiao ZHANG
;
Ying ZHAO
Author Information
1. 中国医科大学附属盛京医院胃肠营养外科,沈阳 110004
- Publication Type:Journal Article
- Keywords:
gastrointestinal stromal tumor;
imatinib mesylate;
sunitinib malate;
combination therapy;
migration;
invasion
- From:
Journal of China Medical University
2025;54(3):193-198
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of imatinib mesylate and sunitinib malate on the migration and invasion of gastroin-testinal stromal tumor(GIST)cells.Methods After identifying primary-cultured GIST cells,their morphology was characterized using atomic force microscopy(AFM).Changes in the expression of genes related to the PI3K/AKT signaling pathway were analyzed using quan-titative real-time PCR following drug treatments.Changes in the binding of related molecules were detected using AFM,and alterations in cell migration,invasive ability,and apoptosis were determined using scratch assay,Transwell assay,and flow cytometry,respectively.Results AFM imaging showed that pseudopods were flatly spread around the GIST cells,indicating characteristics consistent with easy metastasis.Administration of either imatinib or sunitinib significantly reduced the expression of genes related to the PI3K/AKT signaling pathway,the density of epidermal growth factor receptor(EGFR)on the surface of GIST cells,and molecular binding force with EGF.These changes were more pronounced with the combination treatment.Correspondingly,the invasive and migratory abilities of GIST cells were significantly reduced when either drug was administered alone and the inhibitory effect was more significant when the drugs were combined.Conclusion Both imatinib and sunitinib can significantly inhibit the expression of genes related to the PI3K/AKT signaling pathway,reduce the density of EGFR on the surface of GIST cells,and attenuate their molecular binding to EGF,thereby reducing the migration and invasion of GIST cells.However,the combination of these two drugs has a more significant effect.
