Evaluation of chemical constituent consistency in formula granules and traditional decoctions of Gouteng Jiangya Formula
10.3969/j.issn.1001-1528.2025.11.004
- VernacularTitle:钩藤降压方配方颗粒、传统汤剂化学成分一致性评价
- Author:
Qing-gang ZHANG
1
;
Dai-liang ZHANG
;
Hong QI
;
Shu-wen DING
;
Yu-zhuo WANG
;
Yun-lun LI
;
Ji-fu HE
;
Huan-ying GUO
;
Gui-yun CAO
;
Zhao-qing MENG
Author Information
1. 山东中医药大学,山东济南 250355;山东宏济堂制药集团股份有限公司,山东济南 250103
- Publication Type:Journal Article
- Keywords:
Gouteng Jiangya Formula;
formula granules;
traditional decoctions;
chemical constituents;
consistency;
HPLC characteristic chromatograms;
quantitative analysis of multi-components by single-marker(QAMS)
- From:
Chinese Traditional Patent Medicine
2025;47(11):3555-3565
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the chemical constituent consistency in formula granules and traditional decoctions of Gouteng Jiangya Formula.METHODS HPLC characteristic chromatograms were established,the analysis was performed on a 30 ℃ thermostatic YMC-Triart C18 column(4.6 mm× 250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2％phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 240 nm.Puerarin was used as an internal standard to calculate the relative correction factors of 3'-methoxy puerarin,puerarin apioside,magnolflorine,paeoniflora,daidzin,baicalin,palmatine,berberine,wogonoside and benzoylpaeoniflorin,after which the content detemination was made by quantitative analysis of multi-components by single-marker(QAMS).RESULTS The characteristic chromatograms of 9 batches of formula granules and 15 bacthes of traditional decoctions demonstrated the similarities of more than 0.90 at the detection wavelengths of 192,210,240,260,280,300,320,360 nm,along with similar total peak areas.Eleven constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 97.27％-101.64％with the RSDs of 0.36％-1.11％,the result obtained by QAMS and external standard method demonstrated no significant differences(P＞0.05).The contents of various constituents in the formula granules approximated those in the traditional decoctions.CONCLUSION The consistent kinds and contents of various constituents are obversable in formula granules and traditional decoctions of Gouteng Jiangya Formula,which can provide a reference for the reasonable clinical application of this formula.
