Effects of soybean isoflavones on immune function of rats with ulcerative colitis by regulating HMGB1/RAGE signal pathway
10.3969/j.issn.1000-484X.2025.04.011
- VernacularTitle:大豆异黄酮调节HMGB1/RAGE信号通路对溃疡性结肠炎大鼠免疫功能的影响
- Author:
Qifeng XING
1
;
Jinfang ZHOU
1
;
Shiwei ZHANG
1
Author Information
1. 滕州市中心人民医院药学部,滕州 277599
- Publication Type:Journal Article
- Keywords:
Soybean isoflavones;
High mobility group protein B1/receptor for advanced glycation end products signal path-way;
Ulcerative colitis;
Immune function
- From:
Chinese Journal of Immunology
2025;41(4):834-840
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of soybean isoflavones(SIF)on immune function of rats with ulcerative colitis(UC)by regulating high-mobility group protein B1(HMGB1)/receptor for advanced glycation end products(RAGE)signal pathway.Methods:Sixty SD rats were randomly grouped into Model group,low dose SIF group(SIF-L group,300 mg/kg SIF),high dose SIF group(SIF-H group,450 mg/kg SIF),high dose SIF+HMGB1 inhibitor glycyrrhizic acid group(SIF-H+glycyrrhizic acid group,450 mg/kg SIF+30 mg/kg glycyrrhizic acid)and Control group,with 12 rats in each group.The UC rat model was established by TNBS/ethanol combined enema.Body weight changes of rats in each group were measured,and disease activity index(DAI)was determined in each group.Histopathological changes of colon were observed by HE staining;levels of superoxide dismutase(SOD)and malondial-dehyde(MDA)in colon tissue of rats were measured by colorimetry;serum levels of IL-1β,TNF-α and IL-4 were determined by ELISA;flow cytometry was used to determine the percentages of CD3+T,CD4+T and CD8+T lymphocytes in peripheral blood mononuclear cells;levels of serum IgA and IgG were measured by automatic specific protein analyzer;expressions of HMGB1 and RAGE in colon tissues were determined by Western blot and immunohistochemistry.Results:Compared with Control group,levels of DAI,serum TNF-α,IL-1β,IgA,IgG,MDA in colon tissue,HMGB1 and RAGE protein expressions in colon tissue of Model group were obviously higher(P<0.05),while SOD level,IL-4,CD3+T,CD4+T and CD8+T lymphocyte percentages,CD4+/CD8+T were obviously lower(P<0.05),the mucosa of colon tissue was damaged.Compared with Model group,changes of relevant indexes in SIF-H group were contrary to the above(P<0.05),and the damage of colon tissue was alleviated.Conclusion:SIF may enhance the immune function of UC rats by inhibiting HMGB1/RAGE signal pathway.
