Mechanism of IL-23/STAT3/Th17 axis in severe acute pancreatitis and intervention effect of Qingjie Huagong decoction
10.3969/j.issn.1000-484X.2025.04.004
- VernacularTitle:IL-23/STAT3/Th17轴在重症急性胰腺炎中的作用机制及清解化攻方的干预作用
- Author:
Minchao FENG
1
;
Fang LUO
;
Baijun QIN
;
Xiping TANG
;
Kai LI
;
Guozhong CHEN
Author Information
1. 广西中医药大学第一附属医院,南宁 530001;广西中医药防治医学分子生物重点实验室,南宁 530001
- Publication Type:Journal Article
- Keywords:
Qingjie Huagong decoction;
IL-23/STAT3/Th17 axis;
Th17 cell differentiation;
Severe acute pancreatitis;
Immunity
- From:
Chinese Journal of Immunology
2025;41(4):792-797,802
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of IL-23/STAT3/Th17 axis in severe acute pancreatitis(SAP)and the in-terventional effect of the Qingjie Huagong decoction(QJHGD).Methods:A rat model of SAP was established by injecting sodium tau-rocholate into the retrograde pancreatic duct.The blank group,model group,different doses of QJHGD administration groups and posi-tive group were set up respectively.HE staining was used to observe the pathology of pancreatic tissue.ELISA was used to detect serum lipase,α-amylase and inflammatory markers.By combining RT-qPCR,IHC,Western blot,and IF techniques,we elucidated the mechanism of QJHGD in protecting pancreatic tissue in SAP rats.Results:The levels of amylase,lipase,IL-1β,IL-6,IL-17,IL-23,TNF-α and TGF-β in the serum of SAP model rats in all dose groups of QJHGD were significantly reduced,and the effect was the best in medium dose group(P<0.05).The results of IHC and RT-qPCR revealed that the medium-and high-dose groups of QJHGD sig-nificantly reduced the expression of IL-23,STAT3,IL-17 protein and mRNA in the pancreatic tissue of this model(P<0.05).More-over,the Western blot results demonstrated that the expression of IL-23,STAT3,p-STAT3,and IL-17 proteins in SAP rats were sig-nificantly decreased in the medium-dose group of QJHGD(P<0.05);the IF assay indicated that Th17 cell differentiation in SAP rats was inhibited by all dose groups of QJHGD,with the most significant inhibition effect in the middle dose(P<0.05).Conclusion:QJH-GD regulates the activation of IL-23/STAT3/Th17 axis,thereby inhibiting Th17 cell differentiation and exerting a protective effect on pancreatic tissue.
