Effects of Indirect Moxibustion on DNA Methylation of BDNF Promoter Region in the Uterus of Dysmenorrheal Rats
10.19879/j.cnki.1005-5304.202503392
- VernacularTitle:隔物灸对原发性痛经模型大鼠子宫BDNF启动子区DNA甲基化的影响
- Author:
Yanxue XING
1
;
Min ZHOU
;
Xiaoyan WANG
;
Kuo BI
;
Di WANG
;
Zhiguo ZHAO
;
Liyun YANG
;
Xuanping ZHANG
;
Yan ZHANG
;
Fei GAO
;
Wenli LI
;
Xinhua LI
Author Information
1. 河北中医药大学针灸推拿学院,河北 石家庄 050200;河北中医针灸优势病症国际联合研究中心,河北 石家庄 050200
- Publication Type:Journal Article
- Keywords:
primary dysmenorrhea;
indirect moxibustion;
BDNF;
DNA methyltransferase;
epigenetics;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(9):113-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of indirect moxibustion on the expressions of DNA methyltransferases(DNMT)and methylation of the brain-derived neurotrophic factor(BDNF)promoter region in uterine tissues of rats with primary dysmenorrhea(PD);To explore the mechanism of epigenetic regulation of indirect moxibustion on PD model rats.Methods A total of 32 female SD rats were randomly divided into blank group,model group,indirect moxibustion group and Western medicine group,with 8 rats in each group.The PD model with cold dampness stagnation syndrome was established using ice-water baths combined with estradiol benzoate and oxytocin.Starting from the first day of modeling,the indirect moxibustion group received salt-partitioned moxibustion at"Shenque"and ginger-partitioned moxibustion at"Guanyuan"for 20 min,while the Western medicine group was gavaged ibuprofen solution.Both interventions were given once a day for 10 days.On day 11,writhing responses were observed and scored after oxytocin injection,Western blot and RT-qPCR were used to detect protein and mRNA expression of BDNF,DNMT3A and DNMT3B in uterine tissue,immunohistochemical staining was used to detect the positive expressions of DNMT3A and DNMT3B in uterine tissue.The DNA methylation of BDNF promoter region in uterine tissue was detected by sulfite sequencing.Results Compared with the blank group,the writhing latency was shortened and the writhing score increased in the model group(P<0.01);the protein and mRNA expressions of BDNF,DNMT3A and DNMT3B in uterine tissue increased(P<0.01),the positive expressions of DNMT3A and DNMT3B increased(P<0.01),and the DNA methylation rate in BDNF promoter region decreased(P<0.01).Compared with the model group,the writhing latency was lengthened and the writhing score decreased in the indirect moxibustion group and Western medicine group(P<0.05,P<0.01);the protein and mRNA expressions of BDNF,DNMT3A and DNMT3B in uterine tissue decreased(P<0.05,P<0.01),the positive expressions of DNMT3A and DNMT3B decreased(P<0.01),and the DNA methylation rate in BDNF promoter region increased(P<0.01).Conclusion Indirect moxibustion at"Shenque"and"Guanyuan"may inhibit the transcription of BDNF by increasing the DNA methylation level of BDNF promoter region,and reduce the expression of BDNF,so as to relieve the pain of PD rats.
