Abundance and distribution of Staphylococcus aureus isolated from bronchoalveolar lavage fluid of patients with lower respiratory tract infections
10.11816/cn.ni.2025-246943
- VernacularTitle:下呼吸道感染患者肺泡灌洗液金黄色葡萄球菌丰度和分布特征
- Author:
Yaxian JIANG
1
;
Xiaoyu YANG
;
Rui ZHENG
;
Yikun LI
;
Chunrui LI
;
Yuhong GAO
Author Information
1. 云南省第一人民医院检验科,云南 昆明 650032
- Publication Type:Journal Article
- Keywords:
Bronchoalveolar lavage fluid;
Microbial community;
Staphylococcus aureus;
High-throughput sequen-cing;
Sample hierarchial clustering analysis;
Lower respiratory tract infections
- From:
Chinese Journal of Nosocomiology
2025;35(15):2263-2267
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze the microbial community structure in Bronchoalveolar Lavage Fluid(BALF)u-sing high-throughput sequencing technology,with a particular focus on the abundance and distribution of Staphy-lococcus aureus.METHODS A total of 1,650 bronchoalveolar lavage fluid(BALF)samples were collected from the First People's Hospital of Yunnan Province between 2022 and 2023.S.aureus gene detection was performed using BALF culture,isothermal amplification and microfluidic technology.Representative 21 S.aureus-positive samples were grouped into S.aureus pneumonia group,SARS-CoV-2 co-infection group and chronic obstructive pulmona-ry disease(COPD)group,followed by 16S rDNA amplicon high-throughput sequencing.RESULTS The microbial community compositions among the three groups exhibited significant differences.Species-level hierarchical cluste-ring analysis revealed that S.aureus was more frequently detected and accounted for a higher proportion in the S.aureus pneumonia group,while the COPD group was dominated by Achromobacter.In contrast,the SARS-CoV-2 co-infection group was mainly composed of Methylobacterium aquatique and Achromobacter.Only in the S.au-reus pneumonia group was S.aureus the dominant bacterium.CONCLUSION The composition of the BALF micro-biota is closely related to different disease states,providing an important reference for a deeper understanding of the microbiological basis of pulmonary infectious diseases.
