Expression of TCRζ chain and ZAP70 in primary Sj?gren syndrome
10.3969/j.issn.1673-9701.2025.22.004
- VernacularTitle:原发性干燥综合征中TCRζ链及ZAP70的表达
- Author:
Zhen SHI
1
;
Meng HAO
;
Tinghui JIANG-FANG
;
Guohui XUE
;
Le YU
Author Information
1. 九江市第一人民医院检验科,江西九江 332000
- Publication Type:Journal Article
- Keywords:
Primary Sj?gren syndrome;
T cell receptor ζ chain;
Zeta-chain-associated protein kinase 70;
T cell subsets;
Pathogenesis
- From:
China Modern Doctor
2025;63(22):12-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of T cell receptor(TCR)ζchain and Zeta-chain-associated protein kinase 70(ZAP70)in peripheral blood of patients with primary Sj?gren syndrome(PSS).Methods Thirty-six patients with PSS who were treated at Jiujiang NO.1 People's Hospital from January to June 2024 were enrolled in observation group,and 30 healthy subjects during the same period were enrolled in control group.Real-time fluorescent quantitative polymerase chain reaction was used to detect the expression levels of TCRζ chain and ZAP70 in peripheral blood mononuclear cells,and flow cytometry was used to detect peripheral blood T cell subsets.Pearson correlation was used to analyze the correlation between TCRζ chain,ZAP70 and other detection indicators.Results The relative expression levels of TCRζ chain and ZAP70 in observation group were significantly lower than those in control group(P＜0.05),while CD8+and interleukin-6(IL-6)were significantly higher than those in control group(P＜0.05).Pearson correlation analysis showed that TCRζ chain was positively correlated with CD4+,and negatively correlated with CD8+and IL-6(P＜0.05).ZAP70 was negatively correlated with CD8+and IL-6(P＜0.05).Conclusion The expressions of TCRζ chain and ZAP70 are down-regulated in PSS patients,which may exacerbate the immune disorder of PSS through abnormal T cell signal transduction.
