Impact of continuous light exposure on gut microbiota of mice
10.3969/j.issn.1000-4718.2025.02.013
- VernacularTitle:持续光照对小鼠肠道菌群的影响
- Author:
Yan LI
1
;
Lu ZHENG
;
Ru TANG
;
Jian WEI
;
Pei SHI
;
Zhan LI
;
Youqin WANG
Author Information
1. 长治医学院附属和平医院内分泌科,山西 长治 046000
- Publication Type:Journal Article
- Keywords:
continuous light;
circadian clock;
gut microbiota
- From:
Chinese Journal of Pathophysiology
2025;41(2):322-330
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of continuous light on the structure and differential metabolites of gut microbiota in mice.METHODS:The mice were randomly divided into normal light(light/dark,LD)group and 24-hour continuous light(light/light,LL)group.The body weight,fasting blood glucose,serum free fatty acids,serum triacylglycerol and serum total cholesterol levels of each group of mice were measured after 10 weeks.Fresh feces were collected,and 16S rRNA sequencing technology was used to study the effect of continuous light on the diversity,structure,and species composition of gut microbiota in mice.Additionally,liquid chromatography-mass spectrometry(LC-MS)analysis was per-formed to observe the effect of continuous light on the metabolites in mice.RESULTS:Compared with the LD group,the body weight,fasting blood glucose and lipid levels of the LL group were increased(P＜0.05).At the phylum level,the proportion of Firmicutes in the LL group increased,while the proportion of Bacteroidetes decreased.At the class level,the abundance of norank_f_Muribaculaceae and Prevotellaceae_UCG-001 in the LL group decreased significantly,while the abundance of Lactobacillus,Turicibacter and Odoribacter increased significantly.Non-targeted metabolomics analysis iden-tified 65 and 73 differential metabolites under positive and negative modes,involving six major metabolic pathways,in-cluding ABC transporters,purine metabolism,pyrimidine metabolism,secondary bile acid biosynthesis,protein digestion and absorption,and choline metabolism in cancer.CONCLUSION:The structure and metabolites of gut microbiota in mice exposed to continuous light are relatively specific,and inosine may be a key biomarker and potential therapeutic tar-get for biological clock disorders.
