Carthamus tinctorius L.-derived nanovesicles promote endothelial cell au-tophagy through regulating SIPA1L2 ubiquitination
10.3969/j.issn.1000-4718.2025.02.011
- VernacularTitle:红花来源纳米囊泡通过调控SIPA1L2泛素化促进内皮细胞自噬
- Author:
Ye LUO
1
;
Ling YANG
;
Junyu CHEN
;
Jianxin WENG
;
Xiao KE
Author Information
1. 北京大学深圳医院心内科,广东 深圳 518036
- Publication Type:Journal Article
- Keywords:
Carthamus tinctorius L.;
nanovesicles;
autophagy;
SIPA1L2 protein;
ubiquitination
- From:
Chinese Journal of Pathophysiology
2025;41(2):303-310
- CountryChina
- Language:Chinese
-
Abstract:
AIM:This study aimed to investigate the protective effects of Carthamus tinctorius L.-derived nanovesicles(CDNVs)and their regulatory mechanisms in endothelial cell injury induced by oxidized low-density lipopro-tein(ox-LDL).METHODS:Human umbilical vein endothelial cells(HUVECs)were cultured in vitro and subjected to ox-LDL treatment to establish an endothelial cell injury model.The experimental groups included the normal control(NC)group,ox-LDL group(HUVECs treated with 100 mg/L ox-LDL for 24 h),and CDNVs+ox-LDL group(pre-treated with 40 mg/L CDNVs for 1 hour followed by co-culture with 100 mg/L ox-LDL for 24 hours).Cell proliferation and apoptosis were assessed by the EdU incorporation assay and flow cytometry,respectively.Changes in signal-induced proliferation-associ-ated 1-like protein 2(SIPA1L2)mRNA expression were measured by RT-qPCR,and the expression levels of autophagy-related proteins and SIPA1L2 were evaluated by Western blot.SIPA1L2 ubiquitination was evaluated by immunoprecipita-tion assay.RESULTS:(1)CDNVs were successfully isolated and purified,characterized as nanoscale vesicles with a circular shape and a double-layered membrane structure.(2)CDNVs promoted the proliferation and inhibited apoptosis of ox-LDL-treated HUVECs(P＜0.05).(3)CDNVs suppressed the prorein expression of SIPA1L2 and promoted autophagy in ox-LDL-treated HUVECs(P＜0.05).(4)CDNVs facilitated the ubiquitination of SIPA1L2 protein,and reduced its protein level through the ubiquitin-proteasome pathway(P＜0.05).CONCLUSION:CDNVs exert a protective effect against ox-LDL-induced endothelial cell injury by mediating SIPA1L2 ubiquitination and promoting endothelial cell au-tophagy.
