Simultaneous content determination of nine constituents in Chaige Changyuan Mixture by UPLC-MS/MS
10.3969/j.issn.1001-1528.2025.08.002
- VernacularTitle:UPLC-MS/MS法同时测定柴葛畅原合剂中9种成分的含量
- Author:
Long-yan REN
1
;
Liang YANG
;
Xun-rong ZHOU
;
Zhen ZENG
;
Xin DAI
Author Information
1. 贵州中医药大学,贵州贵阳 550025
- Publication Type:Journal Article
- Keywords:
Chaige Changyuan Mixture;
chemical constituents;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2025;47(8):2491-2496
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of isofraxidin,saikosaponin c,ginsenoside Re,puerarin,rosmarinic acid,praeruptorin A,daidzein,baicalin and 5-O-methylvisammioside in Chaige Changyuan Mixture.METHODS The analysis was performed on a 35 ℃ Titank C18 column(100 mm×2.1 mm,3 μm),with the mobile phase comprising of acetonitrile-0.1％formic acid flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.RESULTS Nine constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 84.08％-115.40％with the RSDs of 0.21％-4.47％.CONCLUSION This efficient,simple,sensitive and specific method can be used for the quality control of Chaige Changyuan Mixture.
