Quality evaluation of benchmark sample of Zexie Decoction based on HPLC fingerprints and content determination
10.3969/j.issn.1001-1528.2025.08.001
- VernacularTitle:基于HPLC指纹图谱和含量测定评价泽泻汤基准样品质量
- Author:
Chun-qin LI
1
;
Yue LIANG
;
Yu-juan ZHANG
;
Li-ying PENG
;
Jun-jun SHI
;
An-dong YANG
;
Tuo KAI
Author Information
1. 四川省中医药科学院,中药新药创制川渝共建重点实验室,四川成都 610041
- Publication Type:Journal Article
- Keywords:
Zexie Decoction;
benchmark sample;
quality evaluation;
HPLC fingerprints;
content determination
- From:
Chinese Traditional Patent Medicine
2025;47(8):2485-2490
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of benchmark sample of Zexie Decoction.METHODS HPLC fingerprints were established,after which the content determination of epoxy alisma ene,23-acetyl alisol B,23-acetyl alisol C,alisol A,alisol B,atractylenolide Ⅰ,atractylenolide Ⅱ and atractylenolide Ⅲ was performed,and the transfer rate and paste yield were calculated.RESULTS There were 20 common peaks in the fingerprints for 15 batches of benchmark samples with the similarities of more than 0.95.The average contents of various effective constituents were 180.86 μg/g for alisol B 23-acetate,18.65 μg/g for alisol C 23-acetate,34.74 μg/g for alismoxide,17.65 μg/g for alisol A,238.19 μg/g for alisol B,2.85 μg/g for atractylenolide Ⅰ,6.38 μg/g for atractylenolide Ⅱ,and 15.42 μg/g for atractylenolide Ⅲ,respectively.In the decoction piece-benchmark sample,alisol B 23-acetate,alisol C 23-acetate,atractylenolide Ⅰ,atractylenolide Ⅱ and atractylenolide Ⅲ demonstrated the average transfer rates of 12.09％,16.45％,3.93％,12.17％and 34.37％respectively.The paste yields in various batches of benchmark samples were 15.2％-20.2％.CONCLUSION HPLC fingerprints combined with content determination can be used for the quality control of benchmark sample of Zexie Decoction,thus provides a reference for the development of its compound preparations.
