Berberine alleviates early brain injury after subarachnoid hemorrhage in rats by downregulating NLRP3
10.16557/j.cnki.1000-7547.2024.06.011
- VernacularTitle:小檗碱通过下调NLRP3减轻蛛网膜下腔出血大鼠早期脑损伤
- Author:
Lingyun LIU
1
;
Shujin ZENG
;
Sensen LIN
;
Weijun ZENG
Author Information
1. 湘南学院附属医院,郴州 423000
- Publication Type:Journal Article
- Keywords:
berberine(BBR);
subarachnoid hemorrhage(SAH);
early brain injury(EBI);
NLRP3 inflamma-some;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(6):747-753
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the efficacy and mechanism of berberine(BBR)on early brain injury(EBI)in subarachnoid hemorrhage(SAH)rats.Methods:Sixty-eight SD rats were used to establish SAH model through internal carotid artery puncture and randomly divided into three groups:Sham group,SAH model group,and SAH+BBR group.Rats in the SAH+BBR group were given BBR intragastrically at 3 and 6 h after operation,and were treated with BBR twice on the second day.The severity of SAH grade,neurological score,brain edema and blood-brain barrier func-tion were evaluated 48 h after operation.Neuron apoptosis was detected by immunofluorescence staining.The levels of inflammatory factors in brain tissue were assessed by ELISA.NLRP3 inflammasome,ASC and caspase-1 protein expres-sion were investigated by Western Blot.Results:BBR can promote the recovery of neurological impairment(P＜0.05),improve brain edema and blood-brain barrier function(P＜0.05),reduce neuronal cell apoptosis(P＜0.05),and alleviate brain injury in SAH rats.Compared with SAH group,BBR treatment inhibited the expression of NLRP3 inflammasome,ASC,and caspase-1(P＜0.05).Moreover,BBR decreased the levels of IL-1β,IL-6,and TNF-α in brain tissue(P＜0.05).Conclusion:BBR may reduce the inflammatory response of brain tissue by regulating the acti-vation of NLRP3 inflammasome,thus alleviating the early brain injury after SAH.
