Expression changes of MCHR1 in the dorsal root ganglion of mice under neuropathic pain state
10.16557/j.cnki.1000-7547.2024.06.003
- VernacularTitle:背根神经节MCHR1在小鼠神经病理性疼痛中的表达变化
- Author:
Xia LI
1
;
Zhenzhen LI
;
Wanneng LIU
;
Zhiwei DOU
;
Kexin LIU
;
Ceng LUO
;
Haojun YOU
Author Information
1. 延安大学感觉与运动疾病转化医学研究中心,延安 716000;空军军医大学基础医学院神经生物学教研室,西安 710032
- Publication Type:Journal Article
- Keywords:
neuropathic pain;
spared nerve injury(SNI);
melanin concentration hormone receptor 1(MCHR1);
dorsal root ganglion(DRG);
mouse
- From:
Chinese Journal of Neuroanatomy
2024;40(6):681-687
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of melanin concentration hormone receptor 1(MCHR1)in dorsal root ganglion(DRG)of mice with neuropathic pain.Methods:The expression profile of MCHR1 in the DRG of mice were observed by immunofluorescent staining.Neuropathic pain model was established by spared nerve injury(SNI)in mice.Male mice(C57BL/6)were randomly divided into 2 groups:Sham-operated group and SNI group.The paw withdrawal threshold(PWT)and paw withdrawal latency(PWL)were observed by von Frey fibers and thermal radiation stimulation.The mRNA and protein levels of MCHR1 in DRG were detected by real time RT-PCR and West-ern Blot,respectively.Results:MCHR1 was widely distributed in mouse DRG and co-labeled with small and medium-sized neuronal markers calcitonin gene-related peptide(CGRP)and isolectin B4(IB4),as well as large diameter neu-ronal marker NF200.Stable mechanical hyperalgesia and heat hyperalgesia were observed in the ipsilateral hindpaw of mice at 7 days post-SNI.Real time RT-PCR and Western Blot experiments showed that mRNA and protein expression levels in DRG were both significantly up-regulated in SNI-treated mice,as compared with Sham group.Conclusion:MCHR1 was widely distributed in large,medium and small neurons of DRG.After the SNI model mice presented a sta-ble phenomenon of hyperalgesia,the transcription and protein level of MCHR1 in DRG were significantly elevated in SNI mice.These data suggest that MCHR1 in DRG may be involved in the occurrence and development of neuropathic pain.
