Effect of TINCR-MAF:MAFB transcription factor network on proliferation and differentiation of human kerathnocytes
10.3969/j.issn.1006-5725.2025.04.007
- VernacularTitle:TINCR-MAF:MAFB转录因子网络对人角质形成细胞增殖和分化的影响
- Author:
Jinfen ZHENG
1
;
Cuiping SHI
;
Yunxia LING
;
Dehua ZHANG
;
Qianyu ZHAI
;
Lijia ZHU
;
Doukou JIANG
;
Xiaohong WANG
;
Yonghui LAI
Author Information
1. 深圳市慢性病防治中心皮肤科(广东 深圳 518020)
- Publication Type:Journal Article
- Keywords:
psoriasis;
TINCR;
MAF;
MAFB;
KLF4
- From:
The Journal of Practical Medicine
2025;41(4):509-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the impact of the TINCR-MAF:MAFB transcription factor network on the expression of proliferation and differentiation-related genes in keratinocytes,to verify the role of this network in the occurrence and development of psoriasis and its potential mechanisms.Methods Employed RNA interference technology to knock down TINCR gene expression,and the proliferation ability of keratinocytes was assessed using the CCK-8 method.Additionally,qRT-PCR and Western blot analyses were conducted to evaluate the RNA and protein expression levels of TINCR,MAFB,and KLF4 genes.Immunohistochemical methods were used to detect the expression of KLF4 protein in psoriasis tissues.Results After TINCR gene siRNA interference,the proliferation ability of keratinocytes significantly decreased at 24,48,and 72 hours(P<0.001),indicating that the TINCR gene plays a critical role in cell proliferation.The results of qRT-PCR and Western blot analyses showed that the RNA and protein expression levels of TINCR,MAFB,and KLF4 genes were significantly reduced(P<0.001),suggesting that TINCR may influence the differentiation of keratinocytes by regulating the expression of MAFB transcription factor and KLF4 differentiation-related genes.Furthermore,immunohistochemical results indicated that the expression of KLF4 protein was significantly elevated in psoriasis tissues compared to normal skin tissues,suggesting that KLF4 plays an important role in the pathogenesis of psoriasis.Conclusions The TINCR-MAF:MAFB transcription factor network may participate in the occurrence and development of psoriasis by affecting the proliferation and differentiation of keratinocytes.This finding provides a new perspective on the pathogenesis of psoriasis and potential targets for future therapeutic strategies.
