A comparative study on the efficacy of detection kit based on digital PCR for drug-resistant mutations of mycoplasma pneumonia and tNGS method in detection for common resistance mutation of mycoplasma pneumonia
10.3969/j.issn.1672-8270.2025.11.009
- VernacularTitle:基于数字PCR平台的肺炎支原体耐药突变检测试剂盒与tNGS在肺炎支原体常见耐药突变检测中的效能对比
- Author:
Xiaoxiao WANG
1
;
Xinqiang ZHANG
1
;
Yunhu ZHAO
1
;
Xiaozhen JIANG
1
;
Zongwei CHEN
1
;
Zixia WANG
1
;
Xiuxian CHEN
1
;
Bing GU
1
Author Information
1. 南方医科大学附属广东省人民医院(广东省医学科学院)检验科 广州 510000
- Publication Type:Journal Article
- Keywords:
MicroDrop droplet digital;
Polymerase Chain Reaction(PCR);
Common drug-resistant mutations of mycoplasma pneumonia;
Detection kit for drug-resistant mutation;
Sanger sequencing method;
Targeted next-generation sequencing(tNGS);
Low-frequency mutation
- From:
China Medical Equipment
2025;22(11):61-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the efficacy of the detection kit based on MicroDrop microdroplet digital PCR platform that can identify mycoplasma pneumonia and common drug-resistance mutation,and throughout targeted next-generation sequencing(tNGS)in detecting common drug-resistance mutations of mycoplasma pneumonia.Methods:A total of 300 samples of clinical respiratory tract of pneumonia inpatients at Guangdong Provincial People's Hospital between 2023 and 2024 were collected.Both the detection kit for drug-resistance mutation of mycoplasma pneumoniae and the tNGS method were employed to detect drug-resistance mutation genes.For samples with inconsistent results,Sanger sequencing was used for verification.Results:For the 300 samples,the detection rates of positive mycoplasma pneumonia of the detection kit for drug-resistance mutation of mycoplasma pneumoniae and the tNGS method were respectively 87.00%and 78.67%,with a Kappa value of 0.711,indicating a relatively high level of agreement between the two methods.Among 25 samples with inconsistent results,Sanger sequencing was employed for validation.The results revealed that for samples with low-frequency gene mutations,the reagent kit maintained reliable detection capability,whereas tNGS exhibited missed detections.Thus,the reagent kit demonstrates superior performance in detecting low-frequency mutation samples.Conclusion:The detection rate of low-frequency mutation samples by the digital PCR-based mycoplasma pneumoniae drug-resistance mutation detection kit is higher than that of the tNGS method.This approach helps enhance the accuracy of detection results,providing a rapid and precise means of detecting drug-resistance genes for clinical diagnosis and treatment.
