Rapid on-site detection of 4 kinds of amphetamines in saliva samples using pulsed direct current electrospray ionization mass spectrometry

VernacularTitle:基于pulsed-dc-ESI-MS现场快速检测唾液中的4种苯丙胺类物质
Author: Qiaocui SHI ¹ ; Shengjun CHEN ; Jiayu FU ; Sen YAO ; Weihong XIE
Author Information

1. 浙江省毒品防控技术研究重点实验室,浙江警察学院,浙江杭州 310053
Publication Type:Journal Article
Keywords: Forensic poison analysis; Saliva; Pulsed-dc-ESI-MS; On site rapid detection; Amphetamines
From: Chinese Journal of Forensic Medicine 2025;40(1):70-74
CountryChina
Language:Chinese
Abstract: Objective To develop a method of pulsed direct current electrospray ionization mass spectrometry(pulsed-dc-ESI-MS)for rapid detection of 4 kinds of amphetamines in saliva.Methods Saliva samples were adjusted to optimal pH using NaOH,followed by protein precipitation with anhydrous magnesium sulfate and liquid-liquid extraction with ethyl acetate.The supernatant was then analyzed via pulsed-dc-ESI-MS.Results The limits of detection were 0.01～0.5 ng/mL and the limits of quantitative were 1.0～5.0 ng/mL.In addition,in the concentration range,4 amphetamines have good linear relationships,the correlation coefficients(R2)were all greater than 0.999,the recoveries of 97.21%～104.70%and the relative standard deviations(RSDs)were less than 10%.Conclusion The method has the advantages of simple operation,fast detection,high sensitivity,selectivity and good stability.It can be used for on-site rapid detection of amphetamines in saliva.