Forensic application evaluation of ssDNA library construction-hybridization capture DNA methylation sequencing in height inference
10.13618/j.issn.1001-5728.2025.01.007
- VernacularTitle:单链建库-杂交捕获DNA甲基化测序在身高推断法医学评价
- Author:
Zhonghua WANG
1
;
Qian WANG
1
;
Guangping FU
1
;
Junyan WANG
1
;
Lihong FU
1
;
Bin CONG
1
;
Shujin LI
1
Author Information
1. 河北医科大学法医学院,河北 石家庄 050017
- Publication Type:Journal Article
- Keywords:
Forensic DNA phenotyping;
DNA methylation;
Human height;
Difficult biomaterials
- From:
Chinese Journal of Forensic Medicine
2025;40(1):33-39,55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the sensitivity,repeatability,anti-degradation ability,in vivo temporal stability,and tissue specificity of the DNA methylation sequencing panel constructed in our previous study for height inference,so as to provide a reference for forensic application.Methods Sensitivity:different initial template quantities(50 ng,40 ng,30 ng,20 ng)were set for sequencing.Repeatability:DNA from the same sample was sequenced three times.Anti-degradation ability:whole blood was used to make blood stains,and DNA was extracted and sequenced at 0,3,6 and 9 months,respectively.In vivo temporal stability:the blood was collected at 0,3,6,and 9 months for sequencing.Tissue specificities:published data and findings were used to analyze the tissue specificities of CpGs in the panel.Results The sensitivity test showed that the initial template quantities of 20 ng detected all the CpG sites and still obtained accurate prediction results.The results of the three repeated predictions of the same sample are stable,and the differences are mainly due to the randomness of the DNN model,indicating good detection repeatability.A complete methylation profile was obtained for the blood stains left at room temperature for 9 months,and the predicted results showed a small range of fluctuations.The three samples were predicted to fluctuate within a range of 1.5 cm or less over nine months.Tissue-specific analyses showed a high correlation between blood and saliva,but can not apply to other tissues.Conclusion The DNA methylation detection system we developed in our previous study has good sensitivity,repeatability,anti degradation ability,in vivo time stability,as well as strong tissue specificity,making it suitable for height inference of blood samples.This supports the feasibility of using targeted DNA methylation analysis on whole blood samples to infer height in the field of forensic science.
