Pharmacokinetics of Compound Reserpine and Triamterene in Rats
10.3870/j.issn.1004-0781.2025.02.003
- VernacularTitle:复方利血平氨苯蝶啶在大鼠体内的药动学研究
- Author:
Yifei XIE
1
;
Wen ZHANG
;
Sen ZHANG
;
Junke SONG
;
Haiguang YANG
;
Shoubao WANG
;
Yang LYU
;
Guanhua DU
Author Information
1. 中国医学科学院北京协和医学院药物研究所国家药物筛选中心,药物靶点研究和新药筛选北京市重点实验室,北京 100050
- Publication Type:Journal Article
- Keywords:
Compound reserpine and triamterene;
Pharmacokinetics;
Plasma Concentration;
High performance liquid chromatography-mass spectrometry
- From:
Herald of Medicine
2025;44(2):183-191
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the pharmacokinetics of dihydralazine sulfate,triamterene,hydrochlorothiazide and reserpine in compound reserpine and triamterene in rats.Methods SD rats were randomly divided into three groups.Compound reserpine and triamterene was given at dose of 3.6,10.8 and 32.4 mg·kg-1 by single oral gavage,respectively.HPLC-MS was used to measure the blood concentrations of dihydralazine sulfate,triamterene,hydrochlorothiazide,and reserpine at various time points.DAS software was used to compute the pharmacokinetic parameters.Results After a single oral gavage of 3.6,10.8 and 32.4 mg·kg-1 of compound reserpine tablets triamterene,the tmax of dihydralazine sulfate in rat plasma were 1.50,1.33,and 1.42 h,and the Cmax of dihydralazine sulfate were 12.30,38.31 and 120.52 μg·L-1,respectively.The tmax of triamterene were 1.33,1.33,and 1.42 h,and the Cmax of triamterene were 20.93,67.36,and 168.64 μg·L-1,respectively.The tmax of hydrochlorothiazide were 2.00,2.00,and 1.75 h,and the Cmax of hydrochlorothiazide were 19.89,57.58,and 160.78 μg·L-1,respectively.Risperdal was found at very low levels in rat plasma,and only trace amounts were detected at 1.00 and 1.50 h of 32.4 mg·kg-1 administration.Conclusions Dihydralazine sulfate,triamterene,and hydrochlorothiazide can be eliminated quickly after compound reserpine and triamterene was given orally to rats,and their oral absorption is basically linear.The greater the dosage,the better the absorption of effective components.
