Effect of Zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by L-NAME
10.3969/j.issn.1673-9701.2025.15.001
- VernacularTitle:壮药石崖茶对L-NAME诱导高血压大鼠血压的影响
- Author:
Xuelan LUO
1
;
Weiqian ZHOU
;
Peng YANG
;
Guojun MO
;
Hesheng OU
Author Information
1. 广西中医药大学附属国际壮医医院壮瑶药制剂中心,广西 南宁 530201
- Publication Type:Journal Article
- Keywords:
Adinandra nitida;
N-nitro-L-arginine methyl ester;
Hypertension;
Endothelial nitric oxide synthase;
Nitric oxide
- From:
China Modern Doctor
2025;63(15):1-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Zhuang medicine Adinandra nitida on blood pressure of hypertensive rats induced by N-nitro-L-arginine methyl ester(L-NAME).Methods L-NAME was used to induce hypertension rats model,they were divided into blank group,model group,captopril group,and high,medium,and low dose groups of Adinandra nitida.The rats were continuous gavaged for 4 weeks,and the blood pressures were measured once a week.Rats were anesthetized by intraperitoneal injection,blood samples and tissues were collected.Pathological changes of the rat thoracic aortic tissue were observed by hematoxylin eosin staining,the protein expression level of endothelial nitric oxide synthase(eNOS)in rat thoracic aorta was detected by western blotting,the content of eNOS in rat plasma was measured by enzyme linked immunosorbent assay and nitric oxide(NO)content in rat plasma was detected by one-step reduction method.Results Compared with model group,high and medium dose groups of Adinandra nitida and captopril group of rats showed significant reductions in systolic blood pressure and diastolic blood pressure(P<0.01),while there were no statistically significant change in heart rate(P>0.05).After 4 weeks of oral administration with Adinandra nitida,the damage of the vascular tissues of hypertensive rats induced by L-NAME had been improved,the expression of eNOS protein in thoracic aorta was significantly increased(P<0.01),and the contents of eNOS and NO in rat plasma were significantly increased(P<0.01).Conclusion Zhuang medicine Adinandra nitida has a hypotensive effect on L-NAME induced hypertensive rats,which may be related to the regulation of eNOS and NO.
