Effects of esculin combined with bone marrow mesenchymal stem cell transplantation on the repair of spinal cord injury in rats
10.3969/j.issn.1001-1528.2025.05.011
- VernacularTitle:七叶苷联合骨髓间充质干细胞移植对大鼠脊髓损伤修复的影响
- Author:
Wei-ming YANG
1
;
Chao-lun LIANG
;
Ling CHEN
;
Jin-jin LI
;
Si-lu LIU
;
Kun-rui ZHENG
;
Dian-weng XIE
;
Xing LI
Author Information
1. 广州中医药大学第二临床医学院,广东 广州 510006
- Publication Type:Journal Article
- Keywords:
esculin;
bone marrow mesenchymal stem cells;
spinal cord injury;
oxidative stress;
apoptosis;
Nrf-2/NQO-1 signaling pathway
- From:
Chinese Traditional Patent Medicine
2025;47(5):1486-1493
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the promotional effects of esculin combined with bone marrow mesenchymal stem cells(BM-MSCs)transplantation on the repair of spinal cord injury(SCI)in rats.METHODS The rats were randomly divided into the sham operation group,the model group,the esculin group for gavage of 20 mg/kg esculin,the BM-MSCs group for tail vein injection of 1 mL of 1×106/mL BM-MSCs,and the combinaiton treatment group.The SCI rat model was established using Allen's method,followed by the 14 days consecutive corresponding drug administration starting from the 2nd day after modeling.On days 3,7 and 14 of drug administration,the rats had their hind limbs motor function evaluated by the BBB scoring;and their footprint experiment conducted on the 14th day after modeling.After 14 days of administration,the rats had their morphological changes of spinal cord tissue observed with HE staining and Nissl staining;their activities of SOD and GSH,and level of MDA in spinal cord tissue detected by kits;their expressions of MAP2,GAP43 and GFAP in spinal cord tissue detected by immunofluorescence;and their expressions of NQO-1,Nrf-2,Bcl-2 and Bax proteins in spinal cord tissue detected by Western blot.RESULTS Compared with the model group,the groups interved with esculin,or BM-MSCs,or the combination treatment showed improvements in hind limb function and spinal cord tissue morphology(P＜0.05);decreased MDA levels(P＜0.05);increased SOD and GSH activities(P＜0.05);increased MAP2 and GAP43 fluorescence intensity(P＜0.05);decreased GFAP fluorescence intensity(P＜0.05);increased NQO-1,Nrf-2 and Bcl-2 protein expressions(P＜0.05);and decreased Bax protein expression(P＜0.05).And the combination treatment group was observed with an even better effects(P＜0.05).CONCLUSION The combination of esculin and BM-MSCs transplantation can effectively improve the spinal cord tissue damage and hind limb function in SCI rats.This effect may be achieved by activating the Nrf-2/NQO-1 signaling pathway to inhibit oxidative stress response,thereby reducing neuronal apoptosis,blocking glial scar formation,and promoting stem cell differentiation to rebuild neurons.
