Relationship Between CMTM4 Expression and Clinicopathological Features in Cervical Cancer and the Study of Mechanism
10.13865/j.cnki.cjbmb2025.01.1339
- VernacularTitle:癌症转移相关分子4表达在宫颈癌中与临床病理特征的关系及机制
- Author:
Jian-Hui LIU
1
;
Jing ZHOU
1
;
Hai-Yan WANG
1
;
An-Qi YANG
1
;
Xiao-Ying A
1
;
Jia-Liang WANG
1
;
Qing-Fen MU
1
Author Information
1. 青海大学附属医院妇科,西宁 810001
- Publication Type:Journal Article
- Keywords:
cancer metastasis-related molecule 4(CMTM4);
cervical cancer(CCA);
clinicopathologi-cal features;
proliferation;
apoptosis;
PI3K/AKT
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(2):296-304
- CountryChina
- Language:Chinese
-
Abstract:
Abnormal expression of CMTM4 protein is closely related to tumour occurrence,development and prognosis.Although the important role of CMTM4 in tumours has been gradually manifested,its spe-cific mechanism of action in cervical cancer remains unclear.The aim of this study was to investigate the relationship between CMTM4 expression and clinicopathological features in cervical cancer and study its mechanism.Immunohistochemistry and Western blot were used to detect the expression level of CMTM4 in cancer tissues and paracancerous tissues,and it was found that CMTM4 was significantly under-ex-pressed in cervical cancer tissues(P＜0.001).The chi-square test analysed the relationship between high and low CMTM4 expression and the clinical and pathological characteristics of cervical cancer pa-tients and results found that CMTM4 expression was correlated with the number of births,HPV infection status,pathological type,FIGO stage and lymph node metastasis.Data from Western blot and RT-qPCR found that CMTM4 protein and mRNA levels in HaCaT cells were significantly higher than that of C-33A cells,HeLa cells,U14 cells,and HT-3 cells.Among them,the most significant change in CMTM4 ex-pression was observed in C-33A cells,so the C-33A cell line was selected for subsequent overexpression experiments.CCK-8 analysis found that the proliferation ability of C-33A cervical cancer cells in the pcDNA-CMTM4 group was significantly lower than that in the pcDNA-NC group(P＜0.001).Flow cy-tometry and Western blot results indicated that CMTM4 overexpression promoted apoptosis(P＜0.001),significantly increased Bax(P＜0.001)and cleaved caspase 3(P＜0.05)protein levels,and significant-ly decreased Bcl-2 protein level(P＜0.01).Western blot results further found that CMTM4 overexpres-sion significantly reduced the protein levels of p-PI3K(P＜0.001)and p-AKT(P＜0.01),but did not affect the protein levels of PI3K and AKT(P＞0.05).The above findings indicated that CMTM4 gene expression was down-regulated in cervical cancer,and CMTM4 overexpression inhibited cervical cancer cell proliferation and induced apoptosis by inhibiting the PI3K/AKT pathway.Therefore,CMTM4 may be used as a biological marker for screening cervical cancer.
