Quality evaluation of Gegen Formula Granules
10.3969/j.issn.1001-1528.2025.05.002
- VernacularTitle:葛根配方颗粒质量评价
- Author:
Dai-liang ZHANG
1
;
Chun-xia WANG
;
Lei SHI
;
Yu-kang LIU
;
Yong-qiang LIN
;
Yu-zhuo WANG
;
Jing-hua ZHANG
;
Jin-xin LI
;
Gui-yun CAO
;
Zhao-qing MENG
Author Information
1. 山东中医药大学,山东济南 250355
- Publication Type:Journal Article
- Keywords:
Puerariae lobatae Radix;
formula granules;
standard decoctions;
quality evaluation;
UPLC characteristic chromatograms;
linear calibration with two reference substances(LCTRS);
quantitative determination analysis multi-components by a single marker(QAMS)
- From:
Chinese Traditional Patent Medicine
2025;47(5):1421-1431
- CountryChina
- Language:Chinese
-
Abstract:
AIM To evaluate the quality of Gegen Formula Granules.METHODS Linear calibration with two reference substances(LCTRS)was adopted in the predicting of retention time with puerarin and daidzein as internal standards.UPLC characteristic chromatograms were established.The contents of 3'-hydroxy puerarin,puerarin(internal standard),3'-methoxy puerarin,puerarin 6"-O-xyloside,puerarin apioside and daidzin were determined by quantitative determination analysis multi-components by a single marker(QAMS),after which their transfer rates were calculated.RESULTS Compared with relative retention time method,LCTRS demonstrated higher positional accuracy for characteristic peaks and wider application range for columns.There were 9 characteristic peaks in the characteristic chromatograms for 14 batches of formula granules and 15 batches of standard decoctions with the similarities of more than 0.95.The contents and transfer rates of various constituents in formula granules and standard decoctions were basically consistent.CONCLUSION The chemical constituents in formula granules and their standard decoctions of Puerariae lobatae Radix display good consistency,reliable preparation process is observable in the former.
