EMP1 Induces Proliferation and Migration of Pancreatic Cancer Cells by Activating PI3K/AKT Signaling Pathway
10.3971/j.issn.1000-8578.2025.24.0912
- VernacularTitle:EMP1通过激活PI3K/AKT信号通路诱导胰腺癌细胞增殖和迁移
- Author:
Ru HE
1
;
Xinru LIU
;
Wenkai JIANG
;
Wence ZHOU
Author Information
1. 730030 兰州,兰州大学第二临床医学院;730030 兰州,甘肃省环境肿瘤学重点实验室
- Publication Type:Journal Article
- Keywords:
Pancreatic cancer;
EMP1;
PI3K/AKT
- From:
Cancer Research on Prevention and Treatment
2025;52(3):193-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological behavior of EMP1 in pancreatic cancer cells and the molecular mechanism of EMP1 in promoting tumor progression.Methods A stable EMP1 knockdown cell line was obtained by lentivirus transfection.The effect of EMP1 on the proliferation of cancer cells was determined by CCK-8 and clonal formation assay.The effect of EMP1 on the migration and invasion of cancer cells was detected by scratch test and Transwell test.The influence of EMP1 on downstream signaling pathways was investigated by Western blot.Results The results of qRT-PCR and Western blot showed that EMP1 was highly expressed in pancreatic cancer cells.The results of CCK-8,colony formation,scratch,and Transwell assays indicated that EMP1 promoted the proliferation,migration,and invasion of pancreatic cancer cells.Western blot results revealed that EMP1 might promote tumor progression through the PI3K/AKT signaling pathway.Conclusion This study suggested that EMP1 may activate the PI3K/AKT signaling pathway to promote the proliferation,migration,and invasion of pancreatic cancer cells,thereby positively regulating tumor progression.
