Function of ubiquitin-specific protease 47 in regulating rabies virus infection
10.16303/j.cnki.1005-4545.2025.02.13
- VernacularTitle:泛素特异性蛋白酶47调控狂犬病病毒感染的功能研究
- Author:
Yannan ZHANG
1
;
Mengyang WU
1
;
Chongyang WANG
1
;
Haomiao DONG
1
;
Xin GUO
1
;
Yidi GUO
1
;
Maolin ZHANG
1
Author Information
1. 吉林大学动物医学学院人畜共患传染病重症诊治全国重点实验室/人兽共患病研究所/人兽共患病研究教育部重点实验室,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
rabies virus;
ubiquitin-specific protease 47;
interleukin-6
- From:
Chinese Journal of Veterinary Science
2025;45(2):266-273
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to inquire about the fluctuations of ubiquitin-specific protease 47 on neu-roblastoma cells(Neuro-2a,N2a)infected by rabies virus(RABV).USP47 expression levels were detected after RABV infection in N2a cells through RT-qPCR,protein immunoblotting,and virus titer determination.The levels of RABV nucleoprotein and phosphoprotein gene and protein,and RABV titers in supernatants were analyzed during overexpression and knockdown of USP47.The results showed that RABV infection increased USP47 gene level in N2a cells.When overexpression of USP47,the levels of RABV N and P were increased,and the virus titers were also improved.Mo-reover,the level of interleukin-6(IL-6)genes decreased.Knocking down USP47 expression reduced levels of RABV N and P genes and proteins,lowered the virus titer,and elevated the IL-6 gene lev-el.The results suggest that USP47 promotes RABV infection and suppresses IL-6 expression.This finding lays the foundation for further investigation into the molecular mechanisms by which USP47 regulates RABV infection.
