Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549
10.16352/j.issn.1001-6325.2025.02.0197
- VernacularTitle:敲低DRAM2抑制肺癌细胞系A549的增殖和迁移
- Author:
Haijun LOU
1
;
Zhuoyun TONG
;
Zhenyu ZHANG
;
AHEYERK·MAHESHATI
;
MENG·MENGGEN
;
Muli WUDU
Author Information
1. 新疆医科大学 基础医学院 病理教研室,新疆 乌鲁木齐 830017
- Publication Type:Journal Article
- Keywords:
non-small cell lung cancer;
DRAM2;
p53;
autophagy
- From:
Basic & Clinical Medicine
2025;45(2):197-202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of DNA damage-regulated autophagy factor 2(DRAM2)on the proliferation and migration of non-small cell lung cancer(NSCLC)cells through tumor protein p53(p53)and autophagy.Methods DRAM2 gene was knocked down using lentiviral technology to establish NSCLC cell lines,and autophagy markers were detected by immunofluorescence and Western blot.CCK8 and Transwell assays were used to detect cell proliferation and migration.The effects of autophagy activation and p53 knockdown on autoph-agy and functions of DRAM2?knockdown cells were examined.Results Knockdown of DRAM2 inhibited NSCLC cells,with upregulation of p62 expression(P<0.05)and decreased level of LC3?Ⅱ(P<0.05).Knockdown of DRAM2 suppressed the proliferation(P<0.001)and migration(P<0.001)of NSCLC cells.Activation of auto?phagy partially reversed the inhibitory effects of DRAM2 knockdown on cell proliferation(P<0.01)and migration(P<0.01).When DRAM2 and p53 were knocked down simultaneously,autophagy,cell proliferation(P<0.05)and migration abilities(P<0.001)were restored.Conclusions Knockdown of DRAM2 inhibits the proliferation and migration of lung cancer cell line A549,providing a potential intervention direction for the devel?opment of therapeutic strategies.
