Effects and mechanism of ethanol extract of Angelica sinensis(Oliv.)Diels on cell proliferation inhibition and apoptosis induction in B16-F10 melanoma cells
10.12092/j.issn.1009-2501.2025.01.006
- VernacularTitle:当归乙醇提取物抑制黑色素瘤B16-F10细胞增殖、诱导凋亡的作用及机制研究
- Author:
Jiajie KE
1
;
Yuzhou SHEN
;
Yaping XU
;
Yupei CHEN
;
Peiyuan CHEN
;
Hongtan WU
Author Information
1. 厦门医学院公共卫生与医学技术学院,厦门 361023,福建
- Publication Type:Journal Article
- Keywords:
EEA;
B16-F10 melanoma cells;
cell cy-cle;
apoptosis;
mitochondrial biogenesis and mito-chondrial dynamics
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2025;30(1):51-60
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the effect and mecha-nism of ethanol extract of Angelica sinensis(Oliv.)Diels(EEA)on cell proliferation and apoptosis in B16-F10 melanoma cells.METHODS:Cell viability was analyzed by MTT method.Cell proliferation was detected by colony formation assay.The invert-ed microscope was used to observe the changes of cell growth confluence and morphology.Hoechst 33342 staining was used to detect cell apoptosis.Flow cytometry(FCM)was used to detect cell cycle and apoptosis.Transmission electron microscopy(TEM)was used to observe the changes of cell mi-tochondrial structure.Western blot was used to de-tect the expression levels of cell cycle,apoptosis,mitochondrial biogenesis,and mitochondrial dy-namics-related proteins.RESULTS:Compared with the blank control group,the cell viability of B16-F10 melanoma cells was reduced after EEA(10-400μg/mL)treatment for 24 h and 48 h,respectively(P＜0.05,P＜0.01).The decreased cell growth conflu-ence,morphological changes such as shrinkage,rounding,and reduction in the volume,and apop-totic morphologic changes such as chromatin con-densation were observed after EEA(100 μg/mL and 200 μg/mL)treatment for 24 h.The number of cell clones was decreased after EEA(10-200 μg/mL)treatment for 14 d(P＜0.01).The morphology of mitochondria became more round and shorter,and the inner mitochondrial matrices were either damaged or absent after 200 μg/mL EEA treatment for 24 h.The ratio of cells in G0/G1 phase and the early apoptosis rate of cells were higher than those of the blank control group(P＜0.01)after EEA(20-200 μg/mL)treatment for 24 h.Western blot re-sults showed that compared with the blank control group,the protein expression levels of cleaved cas-pase-9,Bax,DRP1,and FIS1 were up-regulated(P＜0.05,P＜0.01),and the protein expression levels of cyclin D1,cyclin E,CDK2,CDK4,Bcl-2,Bad,Bcl-XL,SIRT1,PGC-1α,NRF1,TFAM,MFN2,and OPA1 were down-regulated(P＜0.05,P＜0.01).CONCLUSION:EEA has an inhibitory effect on the proliferation of B16-F10 melanoma cells,which may be related to the induction of G1/S cell cycle arrest and mito-chondrial apoptotic pathway,and the disruption of mitochondrial biogenesis and mitochondrial dy-namics.
