Inhibitory effects of Shuyu Pills combined with everolimus on TGF-β1-induced epithelial-mesenchymal transition in triple-negative breast cancer cells
10.3969/j.issn.1001-1528.2025.07.009
- VernacularTitle:薯蓣丸联合依维莫司对TGF-β1诱导三阴性乳腺癌细胞上皮间质转化的抑制作用
- Author:
Xiao-shan LUO
1
;
Su XIE
;
Dou-dou FENG
;
Yan-yun MENG
;
Jing-zhe ZHAO
;
Qing XIE
;
Xiang-li LING
Author Information
1. 贵州医科大学,贵州贵阳 550004
- Publication Type:Journal Article
- Keywords:
Shuyu Pills;
everolimus;
triple negative breast cancer;
TGF-β1;
epithelial mesenchymal transformation;
PI3K/Akt/mTOR signaling pathway
- From:
Chinese Traditional Patent Medicine
2025;47(7):2190-2197
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of Shuyu Pills combined with evolimus on the epithelial mesenchymal transformation of triple negative breast cancer cells 4T1 and MDA-MB-231 induced by TGF-β1.METHODS The 4T1 and MDA-MB-231 cells were divided into the blank group and the induction group to induce the epithelial mesenchymal transformation with TGF-β1 cytokine treatment,followed by the assignment into the model group,the Shuyu Pills group,the everolimus group and the Shuyu Pills combined with everolimus group.CCK8 method,plate cloning method,cell scratch test and Transewll test were used to detect the proliferation,cloning formation,migration and invasion ability of the cells whose expressions of E-cadherin,N-cadherin,Vimentin,MMP9,MMP2 and pathway proteins PTEN,PI3K,Akt and mTOR were detected by Western blot.RESULTS Compared with the blank group,the induction group displayed a cell morphological change from epithelioid to stromal,decreased expression of E-cadherin protein(P＜0.01);and increased protein expressions of N-cadherin and Vimentin(P＜0.05).Compared with the model group,each group intervened with the medicine displayed decreased proliferation,clone formation,migration and invasion ability of both kinds of cells(P＜0.01);increased protein expressions of PTEN and E-cadherin(P＜0.05,P＜0.01);and decreased protein expressions of PI3K,Akt,mTOR,N-cadherin,Vimentin,MMP9 and MMP2(P＜0.05,P＜0.01);and more significantly in the Shuyu Pills combined with evolimus group(P＜0.05,P＜0.01).CONCLUSION With a more ideal effect than the single uses in inhibiting the TGF-β1-induced epithelial mesenchymal transformation of triple-negative breast cancer cells,the combination use of Shuyu Pills and everolimus may work through the regulation of PI3K/Akt/mTOR signaling pathway.
