Chemical constituents from Euphorbia humifusa and their in vitro anti-hepatoma activity
10.3969/j.issn.1001-1528.2025.07.017
- VernacularTitle:地锦草化学成分及其体外抗肝癌活性研究
- Author:
Si-fan YAO
1
;
Wu-hui SUN
1
;
Yi ZHANG
1
;
Wen AI
1
;
Xue-jing LI
1
;
Bi-qing ZHAO
1
;
Xiao-jiang ZHOU
1
Author Information
1. 湖南中医药大学,湖南长沙 410208
- Publication Type:Journal Article
- Keywords:
Euphorbia humifusa Willd.;
chemical constituents;
isolation and identification;
anti-hepatoma activity
- From:
Chinese Traditional Patent Medicine
2025;47(7):2243-2249
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Euphorbia humifusa Willd.and their in vitro anti-hepatoma activity.METHODS Silica gel,D101 macroporous adsorption resin and semi-preparative RP-HPLC were used for isolated and purified,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-hepatocellular carcinoma activity was determined by MTT mothod.RESULTS Eighteen compounds were isolated and identified as 22-O-angeloyl-R1-barrigenol(1),dimethyl 3,3'-[oxybis(4,1-phenylene)](2E,2'E)-diacrylate(2),N-(3-methoxy-1,3-dioxopropyl)-D-tryptophan methyl ester(3),N-acetyltryptophan methyl ester(4),N-(methoxycarbonyl)-tryptophan methyl ester(5),(3β,5α,17β)-4,4,8,14-tetramethyl-18-norandrostane-3,17-diol(6),3β,18,19β-trihydroxylupane(7),pregnenolone(8),3-hydroxy-5,6-epoxy-7-megastigmen-9-one(9),dehydrovomifoliol(10),loliolide(11),2,2'-oxybis(1,4-di-tert-butylbenzene)(12),dibutyl phthalate(13),4-methoxycinnamic acid(14),3,4-dimethoxycinnamic acid(15),methyl 4-hydroxybenzoate(16),kaempferol(17),quercetin(18).The IC50 values of compounds 1,7 and 8 on HepG2 cells were(17.27±0.92),(19.11±2.14)and(7.53±1.09)μmol/L,respectively.CONCLUSION Compounds 1-16 are first isolated from this plant.Compounds 1,7 and 8 have anti-hepatoma activity.
