Optimization of extraction process for Bletillae Rhizoma and evaluation of its anti-oxidant,tyrosinase inhibitory activities
10.3969/j.issn.1001-1528.2025.07.006
- VernacularTitle:白及提取工艺优化及其抗氧化、酪氨酸酶抑制活性评价
- Author:
Shan-shan WANG
1
;
Yi-yuan LUO
;
Ye-fei XIE
;
Xin-miao JIANG
;
Ping PAN
;
Kun-ming QIN
;
Hong-jiang CHEN
Author Information
1. 江苏海洋大学药学院,江苏连云港 222005
- Publication Type:Journal Article
- Keywords:
Bletillae Rhizoma;
extraction process;
anti-oxidant activity;
tyrosinase inhibitory activity;
Box-Behnken response surface method
- From:
Chinese Traditional Patent Medicine
2025;47(7):2171-2177
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the extraction process for Bletillae Rhizoma,and to evaluate its anti-oxidant,tyrosinase inhibitory activities.METHODS With ultrasound time,ethanol concentration and solid-liquid ratio as influencing factors,the total extraction content of gastrodin,protocatechualdehyde,p-hydroxybenzaldehyde,1,4-bis[4-(gluconoxy)benzyl]-2-isobutylmalate-2-glucoside,1,4-bis[4-(gluconoxy)benzyl]-2-isobutylmalate,yam Ⅲ,dihydropinosin and 3'-O-methylyam Ⅲ as an evaluation index,the extraction process was optimized by Box-Behnken response surface method.Subsequently,the extract's scavenging effects on DPPH,ABTS+free radicals,and inhibitory ability on tyrosinase were determined.RESULTS The optimal conditions were determined to be 49 min for ultrasound time,55％for ethanol concentration,1∶30 for solid-liquid ratio,and 2 times for extraction frequency,the total extraction content was 13.18 mg/g.The extract demonstrated the IC50 of 10.12,314.07 and 1.70 μg/g on DPPH,ABTS+free radicals and tyrosinase,respectively.CONCLUSION This simple,reliable and stable method can be used for the extraction for Bletillae Rhizoma with strong anti-oxidant,tyrosinase inhibitory activities.
