Polysaccharide from Ganoderma lucidum improves intestinal barrier function and activates Wnt/β-catenin signal pathway in rats with short bowel syndrome
10.13431/j.cnki.immunol.j.20240101
- VernacularTitle:黑灵芝多糖改善短肠综合征大鼠肠屏障功能并激活Wnt/β-catenin信号通路
- Author:
Xiaoxia DONG
1
;
Yun LI
;
Jian CHANG
;
Lin LIU
;
Shufang GUO
;
Jinhong YU
;
Bing DONG
Author Information
1. 056000,邯郸市中心医院营养科
- Publication Type:Journal Article
- Keywords:
Short bowel syndrome;
Intestinal barrier function;
Polysaccharide from Ganoderma lucidum;
Wnt/β-catenin
- From:
Immunological Journal
2024;40(9):727-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impacts of polysaccharide from Ganoderma lucidum(PSG-1)on intestinal barrier function and Wnt/β-catenin signal pathway in rats with short bowel syndrome(SBS).Methods Fifty male SD rats were randomly divided into sham operation group,low dose PSG-1 group(25 mg?kg-1?d-1),medium dose PSG-1 group(50 mg?kg-1?d-1)and high dose PSG-1 group(100 mg?kg-1?d-1).SBS rat model was built,intestinal permeability of rats was detected,intestinal histopathology was observed with HE staining,and the bacterial translocation rate was calculated,the contents of inflammatory cytokines such as interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)were measured by ELISA,immunofluorescence was used to detect the expression and distribution of tight junction protein in ileum,Western blot was used to detect the expression of Wnt and β-catenin.Results Compared with the control group,the villi of ileum in SBS group were sparse,and the villi length,recess depth,Fluorescein isothiocyanate-dextran(FD-40)concentration,bacterial translocation rate(MLN,liver,spleen),levels of IL-6 and TNF-α were significantly increased,the levels of IL-10,Wnt,β-catenin,and the fluorescence densities and protein levels of claudin-1 and occludin were significantly decreased;compared with rats in SBS group,the FD-40 concentration,bacterial translocation rate(MLN,liver,spleen),levels of IL-6 and TNF-α in PSG-1 low,middle and high dose groups were significantly reduced,the villus length,recess depth,the levels of IL-10,Wnt,β-catenin,and the fluorescence densities and protein levels of claudin-1 and occludin were significantly increased.Conclusion PSG-1 may reduce inflammatory reaction and intestinal permeability and improve intestinal barrier dysfunction by activating the expression of Wnt/β-catenin pathway protein in SBS rats.
