4'-Hydroxychalcone attenuates ulcerative colitis by regulating Th17/Treg homeostasis
10.3969/j.issn.1000-4718.2025.01.010
- VernacularTitle:4'-羟基查尔酮通过调节Th17/Treg平衡治疗溃疡性结肠炎
- Author:
Yunyun LIU
1
;
Dongna ZHANG
;
Shanzhi LI
;
Yilong ZHU
;
Ruikang FANG
;
Guangze ZHU
;
Yiquan LI
;
Yuping ZONG
;
Jicheng HAN
Author Information
1. 长春中医药大学中西医结合学院,吉林 长春 130117
- Publication Type:Journal Article
- Keywords:
4'-hydroxychalcone;
ulcerative colitis;
Th17/Treg homeostasis;
JAK/STAT signaling pathway
- From:
Chinese Journal of Pathophysiology
2025;41(1):81-89
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To elucidate the intervention and mechanism of 4'-hydroxychalcone(4-HC)in colitis mice through the regulation of Th17/Treg homeostasis.METHODS:Using a dextran sodium sulfate(DSS)-induced colitis model in mice,we meticulously observed the pathological characteristics of colon tissue via HE staining.Additionally,we employed immunohistochemical analysis and Western blot techniques to assess the expression levels of proteins associated with the JAK/STAT signaling pathway,as well as the specific content of tight junction proteins such as ZO-1 and occludin.The differentiation of Th17 and Treg cells was analyzed through flow cytometry.RESULTS:Compared to the normal group,the DSS group exhibited a consistent decline in body weight,coupled with symptoms of diarrhea and hematochezia,an increase in the DAI score,and a notable reduction in colon length.In contrast,the body weight of the 4-HC group dis-played an upward trend following an initial decrease,with improvements in diarrhea and hematochezia symptoms,a reduc-tion in the DAI score,and a restoration of colon length relative to the model group.The integrity of colon tissue in the 4-HC group was significantly better than that in the DSS group,evidenced by a marked increase in the number of goblet cells and an enhancement in crypt integrity,while the average histology score showed a decrease.Western blot analysis re-vealed substantial increase in ZO-1 and occludin expression after 4-HC treatment.Flow cytometry results indicated a dra-matic decrease in the differentiation rate of Th17 cells in spleen lymphocytes and mesenteric lymph nodes,while the dif-ferentiation rate of Treg cells was significantly elevated.Immunohistochemical and Western blot analyses demonstrated that 4-HC markedly reduced the phosphorylation of STAT1 and STAT3,while up-regulating the phosphorylation of STAT6,suggesting that 4-HC modulates CD4+T cell activity through the JAK-STAT pathway.CONCLUSION:The 4-HC may enhance the course of DSS-induced colitis in mice,alleviate colonic tissue damage,and modulate the balance be-tween Th17 and Treg cells,potentially involving the JAK/STAT signaling pathway.
