Exploring the Mechanism of Moxibustion at"Zusanli"and"Ganshu"Acupoints in Inhibiting Liver Metastasis of Colorectal Cancer Cells in Nude Mice from the Perspective of Gut Microbiota
- VernacularTitle:从肠道菌群探讨艾灸"足三里""肝俞"穴抑制裸鼠结肠癌细胞肝转移机制
- Author:
Yafang SONG
1
;
Xiaomei ZHANG
;
Shiyuan JIANG
;
Yi ZHUANG
;
Yuhang WANG
;
Jianhua SUN
Author Information
- Publication Type:Journal Article
- Keywords: Moxibustion; Intrasplenic injection; Liver metastasis of colorectal cancer; Gut microbiota
- From: World Science and Technology-Modernization of Traditional Chinese Medicine 2024;26(12):3118-3126
- CountryChina
- Language:Chinese
- Abstract: Objective To observe the effect of moxibustion of"Zusanli"and"Ganshu"on the intestinal flora of colorectal cancer cells liver metastasis in nude mice and its possible mechanism was explored.Methods HCT116-GFP colon cancer cells were inoculated by spleen injection,sixty SPF BALB/c male nude mice were divided into model control group,Pre-Mox group,Post-Mox group,Pre-Post-Mox group.Bilateral"Ganshu(BL18)"and"Zusanli(ST36)"acupoints were selected for moxibustion intervention for once a day for 10 minutes each time.the tumor formation rate,liver metastasis rate and the number of tumor of each group were calculated.Furthermore,16S rRNA analysis was used to assess their microbial diversity.Results Moxibustion intervention can significantly reduce the rate of liver metastasis,reduce the number of metastatic foci.Compared with the model control group,Lactobacillus_reuteri and Robinsoniella_peoriensis were significantly lower in Pre-Mox group(P<0.05).Lactobacillus_gasseri decreased significantly,Clostridium_cocleatum and Erysipelatoclostrdum_ramosum multiplex increased significantly in the Post-Mox group(P<0.01).Pre-Post-Mox mice had significantly decreased levels of Lactobacillus_gasseri,and Clostridium_cocleatum,Akkermansia_muciniphila and Clostridium_cocleatum were significantly increased(P<0.05).LEfSe Difference analysis showed that the Pre-Mox group was mainly changed by Coprobacillus;the Post-Mox group was marked by Muribaculaceae and erysipelas;the Pre-Post-Mox group took Verrucomicrobia,Akkermansia_muciniphila and erysipelas as signs.Conclusion Moxibustion could exert an inhibitory effect on CRC liver metastasis,especially the 5-week Pre-PostMox treatment showed the best inhibitory.The potential mechanism might rely on the alteration of microbiota diversity and composition.
