Effects and Mechanism of Polyphyllin Ⅲ on Proliferation,Cycle and Apoptosis of Colon Cancer Cell HCT116
10.19879/j.cnki.1005-5304.202409610
- VernacularTitle:重楼皂苷Ⅲ对结肠癌细胞HCT116增殖、周期、凋亡的影响及其作用机制
- Author:
Yan LUO
1
;
Yilan JIANG
;
Qian ZENG
;
Ling LUO
;
Xuan ZHANG
;
Ji LUO
Author Information
1. 湖南省中西医结合医院(湖南省中医药研究院附属医院),湖南 长沙 410006
- Publication Type:Journal Article
- Keywords:
polyphyllin Ⅲ;
colon cancer;
proliferation;
cell cycle;
apoptosis;
HCT116 cells
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2025;32(6):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of polyphyllin Ⅲ on proliferation,cycle and apoptosis of colon cancer cells HCT116;To explore its mechanism in treating colon cancer.Methods Colon cancer cells HCT116 were cultured in vitro,the cells were dived into control group and polyphyllin Ⅲ low-,medium-and high-dosage group,drug-free media and low-,medium-and high-concentration of polyphyllin Ⅲ were applied to the cells.Cell proliferation was detected by CCK-8 method,and cell cycle and apoptosis were detected by flow cytometry,the expressions of cyclinD1,CDK4,CDK6,RB,p-RB,Bax,Bcl-2,Bcl-xl mRNAs and proteins were detected by RT-PCR and Western blot.Results Compared with the control group,the HCT116 cell viability decreased in the polyphyllin Ⅲ groups with different dosages(P<0.01),the proportion of G0-G1 phase cells increased(P<0.01),the proportion of S,G2-M phase cells decreased(P<0.01),the cell apoptosis rate increased(P<0.01),and the expressions of cyclinD1,CDK4,CDK6,Bcl-2,Bcl-xl mRNA and cyclinD1,CDK4,CDK6,p-RB,Bcl-2,Bcl-xl proteins decreased,while the expression of Bax mRNA and protein increased,with a dosage-dependent manner(P<0.05,P<0.01).Conclusion Polyphyllin Ⅲ can inhibit the proliferation of colon cancer cells cultured in vitro and promote cell apoptosis,blocking cells in the G0-G1 phase.The mechanism may be related to the inhibition of cell cycle related proteins and apoptosis related protein expression.
