Effect of Shenqi Fuzheng injection on apoptosis-related protein expression in nude mice with lung adenocarcinoma xenografts
10.12007/j.issn.0258-4646.2024.12.002
- VernacularTitle:参芪扶正注射液对肺腺癌荷瘤裸鼠移植瘤凋亡相关蛋白表达的影响
- Author:
Bing LIU
1
;
Wenjun LIU
;
Qiuyu ZHAO
;
Jianguang WANG
;
Shengnan GUO
;
Chun WANG
Author Information
1. 辽宁中医药大学中西医结合学院基础药理学教研室,沈阳 110847
- Publication Type:Journal Article
- Keywords:
Shenqi Fuzheng injection;
lung adenocarcinoma xenograft;
apoptosis;
B-cell lymphoma-2-associated X protein;
B-cell lym-poma-2;
caspase-3
- From:
Journal of China Medical University
2024;53(12):1064-1070
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Shenqi Fuzheng injection on the apoptosis of xenograft cells and expression of B-cell lym-phoma-2 (Bcl-2),Bcl-2-associated X protein (Bax),and cleaved caspase-3 proteins in cisplatin-resistant A549/DDP lung adenocarcinoma cells. Methods An A549/DDP xenograft model was constructed using BALB/c-nu nude mice as the research object. Nude mice were treated with normal saline,cisplatin,Shenqi Fuzheng injection,low-dose Shenqi Fuzheng injection combined with cisplatin,or high-dose Shenqi Fuzheng injection combined with cisplatin to observe xenograft growth. Ultrastructural changes in the tumor cells in each group were observed using transmission electron microscope (TEM) . Apoptotic cells were visualized using the terminal deoxynucleotidyl trans-ferase dUTP nick end labeling (TUNEL) assay. The expression of the apoptosis-related proteins Bax,Bcl-2,and cleaved caspase-3 in the xenograft tissue was measured using Western blotting and immunohistochemistry (IHC) . Results Compared with the cisplatin group,the tumor volume was significantly reduced in the combination groups (P<0.05) . The TEM results showed that,compared with the cisplatin group,the cells in the cisplatin combined with Shenqi Fuzheng injection group exhibited overall edema,severe shrinkage,chromosomal aggregation,and a specific apoptotic morphology. TUNEL staining showed that,compared with the cisplatin group,the apoptotic rate of the cisplatin combined with Shenqi Fuzheng injection group increased (P<0.05) . The IHC and Western blotting results showed that the cleaved caspase-3 and Bax expression levels were significantly higher in the combination group than in the cisplatin group,whereas Bcl-2 expression was down-regulated (P<0.05) . Conclusion Shenqi Fuzheng injection strengthens the antitumor sensitivity of cisplatin,and its mechanism of action may be related to the upregulation of Bax,downregulation of Bcl-2,and activation of cleaved caspase-3 to induce apoptosis.
