Protective effects of several antioxidants on porcine Sertoli cells injury induced by fumonisin B1
10.16303/j.cnki.1005-4545.2024.12.14
- VernacularTitle:几种抗氧化物对伏马毒素B1诱导猪支持细胞损伤的保护作用
- Author:
Ruixue HUANG
1
;
Jun MA
;
Huai ZHANG
;
Xiaodong DONG
;
Luoyu MO
;
Jian LI
;
Shi YIN
Author Information
1. 西南民族大学 畜牧兽医学院,四川成都 610041;西南民族大学 现代生物技术国家民委重点实验室,四川成都 610041
- Publication Type:Journal Article
- Keywords:
fumonisin B1;
porcine Sertoli cell;
oxidative stress;
pterostilbene;
quercetin;
curcumin
- From:
Chinese Journal of Veterinary Science
2024;44(12):2597-2604
- CountryChina
- Language:Chinese
-
Abstract:
In this study,porcine Sertoli cells were selected as the research subjects to investigate the protective effects and mechanisms of three antioxidants:pterostilbene(PTE),quercetin(QR),and curcumin(CUR)against fumonisin B1(FB1)-induced Sertoli cell injury in pigs.The results showed that after treatment with different concentrations of FB1(0,20,40,and 80 μmol/L),the viability of Sertoli cells significantly decreased,while the intracellular levels of reactive oxygen spe-cies(ROS)significantly increased,with 80 μmol/L FB1 exhibiting the most pronounced effect.The addition of QR to Sertoli cells treated with 80 μmol/L FB1 significantly improved cell proliferation and inhibited apoptosis,with QR demonstrating the most effective results.Concurrently,the ex-pression levels of several genes related to proliferation and apoptosis,including Bax,Bcl-2,Caspase-3 and PCNA,changed significantly following the addition of the three antioxidants.After FB1 treatment,the contents of reactive oxygen species(ROS)and malondialdehyde(MDA)were significantly increased,while the activity of superoxide dismutase(SOD)and the expression of some antioxidant enzyme genes were significantly down-regulated.The contents of ROS and MDA in porcine Sertoli cells significantly decreased following the addition of three kinds of antioxidant and QR was most effective.At the same time,SOD activity was significantly up-regulated in the Sertoli cells of both mice and pigs treated with the three kinds of antioxidant.The expression of an-tioxidant genes,including SOD1,CAT,GPX1,and PRDX1,exhibited significant changes at the mRNA level.Following FB1 treatment,the integrity of the mitochondrial membrane in porcine Sertoli cells was compromised,leading to a reduction in mitochondrial membrane potential.Howev-er,the addition of the three antioxidants partially restored the mitochondrial membrane potential.The findings of this study provide a theoretical foundation for the prevention and treatment of FB1-induced reproductive toxicity in livestock and poultry,ultimately enhancing the reproductive performance of male animals.
