Application and progress of CRISPR/Cas9 strategies(delivery)in bron-chial epithelial cells
10.3969/j.issn.1000-4718.2024.00.029
- VernacularTitle:CRISPR/Cas9技术(递送)在支气管上皮细胞中的应用和进展
- Author:
Yingying ZHANG
1
;
Chuwen WANG
;
Guoqing QIAN
Author Information
1. 宁波大学医学部,浙江 宁波 315211
- Publication Type:Journal Article
- Keywords:
CRISPR/Cas9 system;
bronchial epithelial cells;
gene editing
- From:
Chinese Journal of Pathophysiology
2025;41(1):173-180
- CountryChina
- Language:Chinese
-
Abstract:
Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology is easy to operate,efficient,and applicable,and has unique advantages in probing the underlying mechanisms of the airways.Bronchial epithelial cells form a physical barrier of lung defense,and their damage or dysfunc-tion involves in the pathogenesis of chronic airway diseases.In recent years,the incidence of chronic airway diseases has increased,but the progress of treatment has been relatively slow,and there is an urgent need to find a therapeutic break-through.The CRISPR/Cas9 system enables precise gene editing and provides a new strategy for chronic airway diseases.In this review,we provide an overview of the application and progress of CRISPR/Cas9 in primary or immortalized bronchial epithelial cells and in vivo animal models in terms of its delivery mode and mechanism,and discuss the development pros-pects and challenges of CRISPR/Cas9,to provide potential future role of this strategy for airway disease treatment.
